전뇌허혈 유발 후 침전극저주파자극 적용이 소뇌의 세포자멸사 억제에 미치는 영향 = The Effect of Needle Electrode Electrical Stimulation Following Global Ischemia on the Suppression of Apoptosis in the Cerebellum

국문 초록 (Abstract)

본 연구는 온목동맥의 일시적 폐색에 의해 유발된 전뇌허혈로 인해 발생한 SD 랫드 소뇌 부위의 세포자멸사에 대한 침전극저주파자극(Needle Electrode Electrical Stimulation : NEES) 적용의 효과를 알아보기 위하여 면역조직화학적검사법을 통하여 조직을 관찰하였다. 연구를 통하여 대조군과 전뇌허혈유발군, 침전극저주파자극시행군의 세포자멸사 관련 인자의 발현정도를 비교하여 분석한 결과 전뇌허혈 후 침전극저주파의 적용은 SD 랫드 소뇌 부위의 c-fos 발현 감소에 유의한 효과를 보였으며, bax, caspase-3의 발현 감소에는 유의한 영향이 없는 것으로 나타났다. 이상의 결과로 보아 전뇌허혈 후 침전극저주파자극의 적용은 세포자멸사 관련 인자의 발현 억제에 일부분 효과성이 있는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

This study conducted observation of tissues using the immunohistochemistry methods in order to see the effect of Needle Electrode Electrical Stimulation(: NEES) on apoptosis in the SD rat cerebellum caused by global ischemia which came from common carotid artery occlusion. The comparison and analysis results of expression of factors related to apoptosis among the control group, the global ischemia group and the NEES group showed that NEES following global ischemia had a significant effect on the reduction of c-fos expression in the SD rat cerebellum, and didn't have a significant effect on the reduction of bax and caspase-3 expression. In conclusion, it is considered that NEES following global ischemia is partially effective in suppressing the expression of factors related to apoptosis.

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