12주간 지구성 트레드밀 운동이 고혈압쥐 대동맥의 Peroxisome proliferator activated receptor γ발현에 미치는 영향

국문 초록 (Abstract)

윤아람, 박희근, 이영란, 권순미, 장학영, 이위, 이상기, 이왕록, 전종귀. 12주간 지구성 트레드밀 운동이 고혈압 쥐 대동맥의 Peroxisome proliferator activated receptor γ 발현에 미치는 영향. 운동과학, 제21권 제3호. 355-364, 2012. 본 연구의 목적은 12주간 지구성 트레드밀 운동이 고혈압 쥐 대동맥의 PPARγ 발현에 미치는 영향을 살펴보고 이와 관련된 염증성 사이토카인과 항산화 단백질 발현의 변화를 살펴 보기 위하여 실시하였다. 실험동물은 정상혈압 통제군(WKY control; WC), 정상혈압 운동군(WKY exercise; WE), 고혈압 통제군(SHR control; SC), 고혈압 운동군(SHR exercise; SE) 4집단으로 구성하고, 각 집단 간 8마리씩 무선배정 하였다. 지구성 운동 집단은 16m/min, 0 % grade, 5일/주, 30~60분간 총 12주 동안 트레드밀 운동을 실시하였다. 마지막 운동 24시간 후 모든 집단을 희생시키고, PPAR-γ, MCP-1, TNF-α, IL-1β, Mn-SOD, HSP70 단백질의 발현을 측정하였다. 대동맥에서 PPAR-γ 단백질의 발현은 정상혈압과 고혈압군 모두 운동에 의해 증가되었다. MCP-1과 TNF-α는 고혈압군에서 만 감소되는 것으로 나타났으며 IL-1β는 그룹 간 유의한 차이가 없었다. Mn-SOD, HSP70은 정상혈압과 고혈압군 모두 운동에 의해 증가되는 것으로 나타났다. 따라서 12주간의 지구성 운동은 고혈압 쥐의 대동맥에서 PPAR-γ 발현을 증가시킴에 따라 염증성 사이토카인의 발현을 억제하고 항산화 단백질의 발현을 증가시켜 혈압을 낮추는데 중요한 역할을 할 수 있음을 시사한다.

다국어 초록 (Multilingual Abstract)

Yoon, A. R., Park, H. G., Lee, Y. R., Kwon, S. M., Jang, H. Y., Li, W., Lee, S. K., Lee, W. L., Jun, J. K. The Effect of 12 weeks of Exercise Training on Peroxisome proliferator activated receptor γ in Aorta of Hypertensive Rats. Exercise Science, 21(3): 355-364. 2012. The purpose of this study was to investigate the expression of PPAR-γ, inflammatory cytokines and antioxidant proteins in aorta by 12 weeks of endurance exercise training, The Wistar-Kyoto rats (WKY, n=16) and Spontaneously hypertensive rats (SHR, n=16) were divided into 4 groups; WKY control (WC), WKY exercise (WE), SHR control (SC), SHR exercise (SE), and submitted to treadmill exercise for 12 weeks (5 days/week, 30~60 min/day), Every other weeks, body weight and systolic blood pressure were measured. 24 hours after last exercise, all rats were sacrificed then, blood lipid profiles were measured and the expression of PPAR-γ, inflammatory cytokines and antioxidant proteins were analyzed by western blotting. The expression of PPAR-γ of WE and SE were significantly increased compared with WC and SC. MCP-1 and TNF-α were significantly decreased in SE, but IL-1β shows no differences, Mn-SOD and HSP70 were significantly increased by exercise in WE and SE. Therefore, we suggested that endurance exercise could increase the PPARγ expres sion and decrease the inflammatory cytokines and increase antioxidant proteins and it plays a important role in ameliorating the hypertension.

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