케어스웰을 이용한 사이토카인 억제 효과에 대한 연구 = The Study of Cytokine Inhibitory Effect using Careswell

다국어 초록 (Multilingual Abstract)

Objectives : In this study, we attempted to evaluate the effects of Careswell on human mast cell-mediated allergy inflammation in vitro and pruritogen-induced scratching behavior in vivo. Method : The Careswell was extract by distilled water. The anti-itching effects of Careswell were investigated on the compound 48/80 (50 μg/kg) or histamine (100 μg/kg) induced scratching behavior male ICR mice for 30 min by an observer blind. Terfenadine (10 mg/kg) was used as a positive control drug. The cell toxicity of Careswell was determined by 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The regulatory effect of Careswell on interleukin (IL)-6 and tumor necrosis factor (TNF) -α levels was determined by enzyme-linked immunosorbent assay (ELISA) in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) stimulated human mast cells (HMC-1). Also, we evaluated the effect of Careswell on PMACI induced the activation of Nuclear factor-kappa B (NF-κB) into nucleus by Western blot analysis. Result : The results revealed that the oral administration of Careswell (200 mg/kg, p.o.) attenuated the compound 48/80 or histamine-induced scratching behavior in mice. We showed that Careswell significantly reduced the PMACI-induced the production of IL-6 (0.5-1 mg/ml) and TNF-α (0.1-1 mg/ml). Additionally, Careswell significantly inhibited the activation of NF-κB in PMACI-stimulated HMC-1. Conclusion : Collectively, the findings of this study provide us with a novel insight into the pharmacological actions of Careswell as a potential molecule for use in the treatment of allergic inflammation diseases.

참고문헌 (Reference)

1 김현희, "알레르기비염 및 부비동염과 천식의 연관성" 대한소아과학회 50 (50): 335-339, 2007

2 안강모, "비만세포의 알레르기 연증 반응과 Innate Immunity에서의역할" 대한소아과학회 47 (47): 1137-1141, 2004

3 정진기, "북강활(北羌活) 물추출물의 항알레르기 효능에 대한 연구" 대한본초학회 25 (25): 35-41, 2010

4 Inami Y, "Topical surfactant-induced pruritus: involvement of histamine released from epidermal keratinocytes" 344 (344): 459-466, 2013

5 Papanicolaou DA, "The pathophysiologic roles of interleukin-6 in human disease" 128 (128): 127-137, 1998

6 Brown JM, "The mast cell and allergic diseases: role in pathogenesis and implications for therapy" 38 (38): 4-18, 2008

7 Baldwin AS Jr, "The NF-kappaB and I kappaB proteins：new discoveries and insights" 14 : 649-683, 1996

8 Yamamoto Y, "Sulindac inhibits activation of the NF-kappa B pathway" 274 (274): 27307-27314, 1999

9 Azzolina A, "Substance P induces TNF-alpha and IL-6 production through NF kappa B in peritoneal mast cells" 1643 (1643): 75-83, 2003

10 Chen F, "New insights into the role of nuclear factor-kappaB, a ubiquitous transcription factor in the initiation of diseases" 45 : 7-17, 1999

11 Barnes PJ, "NF-kappa B: a pivotal role in asthma and a new target for therapy" 18 (18): 46-50, 1997

12 Cook EB, "Mechanisms of antihistamines and mast cell stabilizers in ocular allergic inflammation" 1 (1): 167-180, 2002

13 Harvima IT, "Mast cells as regulators of skin inflammation and immunity" 91 (91): 644-650, 2011

14 Galli SJ, "Mast cells as "tunable"effector and immunoregulatory cells: recent advances" 23 : 749-786, 2005

15 Gilmore TD, "Inhibition of NF-κB signaling as a strategy in disease therapy" 349 : 245-263, 2011

16 Bradding P, "Immunopathology and human mast cell cytokines" 31 (31): 119-133, 1999

17 Seoul National University College of Medicine, "Immunology" Seoul National University publisher 188-197, 1987

18 Bunikowski R, "Effect of low-dose cyclosporin a microemulsion on disease severity, interleukin-6, interleukin-8 and tumor necrosis factor alpha production in severe pediatric atopic dermatitis" 125 (125): 344-348, 2001

19 Kim SH, "Effect of Dracocephalum argunense on mast cell mediated hypersensitivity" 139 (139): 87-95, 2006

20 Xue CC, "Does acupuncture or Chinese herbal medicine have a role in the treatment of allergic rhinitis?" 6 (6): 175-179, 2006

21 Trefzer U, "Cytokine and anti-cytokine therapy in dermatology" 3 : 733-743, 2003

22 Nilsson G, "Constitutive and inducible cytokine mRNA expression in the human mast cell line HMC-1" 42 (42): 76-81, 1995

23 Minardi T, "Complementary and alternative medicine : Herbs, phytochemicals and vitamins and their immunologic effects" 123 : 283-294, 2009

24 Korean Journal of Asthma, Allergy and Clinical Immunology, "Asthma and Allergy disease" koonja publisher 66-, 2002

25 Church MK, "Allergy and allergic diseases. 1st ed" Blackwell Science 149-170, 1997

26 Gadaleta RM, "Activation of bile salt nuclear receptor FXR is repressed by pro-inflammatory cytokines activating NF-κB signaling in the intestine" 1812 (1812): 851-858, 2011