먹물버섯 Coprinus comatus를 yeast extract peptone dextrose 액체배지에서 배양하고 laccase의 생성 분비를 확인하였다. 이 때 분비된 laccase를 이온교환수지 방법과 전기영동 분획수집방법으로 순수하게 ...
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https://www.riss.kr/link?id=A103312685
김수연 (강원대학교) ; 최지영 (강원대학교) ; 최형태 (강원대학교) ; Kim, Su Yeon ; Choi, Ji Young ; Choi, Hyoung T.
2017
Korean
KCI등재,SCOPUS
학술저널
79-82(4쪽)
1
0
상세조회0
다운로드국문 초록 (Abstract)
먹물버섯 Coprinus comatus를 yeast extract peptone dextrose 액체배지에서 배양하고 laccase의 생성 분비를 확인하였다. 이 때 분비된 laccase를 이온교환수지 방법과 전기영동 분획수집방법으로 순수하게 ...
먹물버섯 Coprinus comatus를 yeast extract peptone dextrose 액체배지에서 배양하고 laccase의 생성 분비를 확인하였다. 이 때 분비된 laccase를 이온교환수지 방법과 전기영동 분획수집방법으로 순수하게 정제하였다. 정제된 효소단백질의 분자량은 SDS-PAGE로 분석하였을 때 약 67 kDa으로 나타났고 최적산도는 pH 4.3, 최적온도는 $25^{\circ}C$로 확인되었다. 기질 o-tolidine에 대한 Km 값은 0.45 mM, Vmax 값은 0.0132 OD/min/unit로 나타났다. 정제된 laccase를 사용하여 염료 RBBR과 Poly R-478의 탈색을 분석한 결과 배양 12시간 후에 RBBR은 49.3% 탈색되었고 poly R-478은 41.6% 탈색되었다.
다국어 초록 (Multilingual Abstract)
An inky cap, Coprinus comatus synthesizes and secretes a laccase in the liquid yeast extract peptone dextrose medium. We have successfully purified the enzyme through the ion-exchange chromatography and the preparative gel electrophoresis. The estimat...
An inky cap, Coprinus comatus synthesizes and secretes a laccase in the liquid yeast extract peptone dextrose medium. We have successfully purified the enzyme through the ion-exchange chromatography and the preparative gel electrophoresis. The estimated molecular weight was 67 kDa by the SDS-PAGE analysis. Optimum pH was pH 4.3 and optimum temperature was $25^{\circ}C$. The Km value was 0.45 mM and the Vmax was 0.0132 OD/min/unit for o-tolidine. Purified laccase showed 49.3% decolorizing activity against remazol brilliant blue R (RBBR) and 41.6% decolorizing activity against Poly R-478 after 12 h incubation.
참고문헌 (Reference)
1 강애리, "Trametes versicolor에 의한 Bisphenol A 생분해의 최적조건" 한국미생물학회 44 (44): 37-42, 2008
2 Kim, Y, "Removal of estrogenic activity from endocrine disrupting chemicals by purified laccase of Phlebia tremellosa" 284 : 172-175, 2008
3 Wang, S. S, "Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01" 95 : 920-927, 2017
4 Ko, E. M, "Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum" 57 : 98-102, 2001
5 Choi, Y. O, "Purification and characterization of laccase from Coprinu congregates" 21 : 351-358, 1995
6 Shrestha, P, "Isolation and physicochemical characterization of laccase from Ganoderma lucidum-CDBT1 isolated from its native habitat in Nepal" 2016
7 Bao, S, "Heterologous expression and characterization of a novel lacase isoenzyme with dyes decolorization potential from Coprinus comatus" 40 : 1927-1936, 2013
8 Kum, H, "Dye removal by Phlebia tremellosa transformants with lignin degrading enzymes" 46 : 93-95, 2010
9 Jiang, M, "Decolorization of synthetic dyes by crude and purified laccases from Coprinus comatus grown under different cultures: the role of major isoenzyme in dyes decolorization" 169 : 660-672, 2013
10 Kim, H, "Decolorization of remazol brilliant blue R by a purified laccase of Polyporus brumalis" 166 : 159-164, 2012
1 강애리, "Trametes versicolor에 의한 Bisphenol A 생분해의 최적조건" 한국미생물학회 44 (44): 37-42, 2008
2 Kim, Y, "Removal of estrogenic activity from endocrine disrupting chemicals by purified laccase of Phlebia tremellosa" 284 : 172-175, 2008
3 Wang, S. S, "Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01" 95 : 920-927, 2017
4 Ko, E. M, "Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum" 57 : 98-102, 2001
5 Choi, Y. O, "Purification and characterization of laccase from Coprinu congregates" 21 : 351-358, 1995
6 Shrestha, P, "Isolation and physicochemical characterization of laccase from Ganoderma lucidum-CDBT1 isolated from its native habitat in Nepal" 2016
7 Bao, S, "Heterologous expression and characterization of a novel lacase isoenzyme with dyes decolorization potential from Coprinus comatus" 40 : 1927-1936, 2013
8 Kum, H, "Dye removal by Phlebia tremellosa transformants with lignin degrading enzymes" 46 : 93-95, 2010
9 Jiang, M, "Decolorization of synthetic dyes by crude and purified laccases from Coprinus comatus grown under different cultures: the role of major isoenzyme in dyes decolorization" 169 : 660-672, 2013
10 Kim, H, "Decolorization of remazol brilliant blue R by a purified laccase of Polyporus brumalis" 166 : 159-164, 2012
11 Laemmli,U.K, "Cleavage of structural proteins during assembly of the head of bacteriophage T4" 227 : 680-685, 1970
12 Zhao, S, "A novel laccase with potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from mycelia of mushroom Coprinus comatus" 2014
Draft genome sequence of Lactobacillus salivarius KLW001 isolated from a weaning piglet
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2013-12-02 | 학술지명변경 | 외국어명 : The Korean Journal of Microbiology -> Korean Journal of Microbiology | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2001-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
1998-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.21 | 0.21 | 0.21 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.26 | 0.24 | 0.48 | 0.02 |