3-Methyl-1-phenyl-5-pyrazolone 유도체화와 UPLC-TOF/MS를 이용한 괭생이모자반 유래 푸코이단의 단당류의 조성 분석 = Analysis of momosaccharide composition of fucoidan from Sargassum horneri using UPLC-DAD-TOF/MS combined with precolumn derivatization by 3-methyl-1-phenyl-5-pyrazolone

다국어 초록 (Multilingual Abstract)

The simple, effective, and sensitive method for analyzing monosaccharide composition of fucoidan using UPLC-DAD-TOF/MS combined with precolumn derivatization by 3-methyl-1-phenyl-5-pyrazolone (PMP) reagent. This method is used to identify and quantify the monosaccharide composition in fucoidan from Sargassum horneri. The UPLC method with PMP precolumn derivatization is reported (simple, rapid, convenient and reproducible) and it also proved to be suitable for the UPLC analysis of fucoidan. Also, this method can be used for determination of monosaccharide contents in other seaweed polysaccharides by changing the pH value of the mobile phase. In order to ensure reliability, this analytical method was validated by analyzing the linearities, precisions, and recovery rates of mixed standard solutions of mannose and other monosaccharides. Fucoidan derived from S. horneri is composed of mannose, glucose, galactose, xylose, glucuronic acid, rhamnose and fucose, with the fucose content found to exceed 50% of the fucoidan composition. The results of this study help to structurally analyze the fucoidan from S. horneri. It is expected that the analytical method established in this study will be useful for the standardization and development of functional raw materials from S. horneri.

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