Pb2+이 청개구리 배아 발달에 미치는 영향

다국어 초록 (Multilingual Abstract)

The Frog Embryo Teratogenesis Assay-Xenopus(FETAX) protocol has recently been adopted as a valuable tool for evaluating the embryotoxicity of chemical toxicity and environmental contaminants in frogs. In this experiment, embryotoxicity of the Pb2+ was determined in Korean frog, Hyla japonica using the FETAX protocol. The percentage of mortality as well as the percentage of malformed larvae was investigated by probit analysis. The 96-h LC50 of 0.005 ㎎/ℓ and EC50 of 0.003 ㎎/ℓ, a TI of 1.67 was derived; indicating that Pb2+ is to be considered a teratogenic compound.
To examine the reversibility of the inhibitory effects, the embryos were exposed to the Pb2+ only for 24 hours and transferred to plain medium and cultured further for 48 hours. The embryos were recovered from the toxic effect of Pb2+ when they were exposed to 0.005, 0.01㎎/ℓ of Pb2+ for 24hours, but not to 0.5㎎/ℓ of Pb2+.
From the above results, it reveal that Pb2+ in this experiment suppressed the development of embryos at relatively low concentration. Therefore, the Hyla japonica embryo teratogenesis assay system can be used as a useful tool to evaluate the toxicity of the pollutants in environment.

목차 (Table of Contents)

• Ⅰ. 서 론 375
• Ⅱ. 재료 및 방법 376
• Ⅲ. 결 과 377
• Ⅳ. 논 의 381
• 참고문헌 382
• ABSTRACT 384