Transcriptome Analysis of Induced Systemic Drought Tolerance Elicited by Pseudomonas chlororaphis O6 in Arabidopsis thaliana

다국어 초록 (Multilingual Abstract)

Root colonization by Pseudomonas chlororaphis O6induces systemic drought tolerance in Arabidopsis thaliana.
Microarray analysis was performed using the 22,800-gene Affymetrix GeneChips to identify differentiallyexpressed genes from plants colonized with or without P. chlororaphis O6 under drought stressed conditions or normal growth conditions. Root colonization in plants grown under regular irrigation condition increased transcript accumulation from genes associated with defense,response to reactive oxygen species, and auxin- and jasmonic acid-responsive genes, but decreased transcription factors associated with ethylene and abscisic acid signaling. The cluster of genes involved in plant disease resistance were up-regulated, but the set of drought signaling response genes were down-regulated in the P.
chlororaphis O6-colonized under drought stress plants compared to those of the drought stressed plants without bacterial treatment. Transcripts of the jasmonic acid-marker genes, VSP1 and pdf-1.2, the salicylic acid regulated gene, PR-1, and the ethylene-response gene,HEL, also were up-regulated in plants colonized by P.
chlororaphis O6, but differed in their responsiveness to drought stress. These data show how gene expression in plants lacking adequate water can be remarkably influenced by microbial colonization leading to plant protection,and the activation of the plant defense signal pathway induced by root colonization of P. chlororaphis O6 might be a key element for induced systemic tolerance by microbes.

참고문헌 (Reference)

1 Hajgaard, J., "Two antifungal thaumatinlike proteins from barley grain" 291 : 127-131, 1991

2 Hajgaard, J., "Two antifungal thaumatinlike proteins from barley grain" 291 : 127-131, 1991

3 Cartieaux, F., "Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance" 36 : 177-188, 2003

4 Cartieaux, F., "Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance" 36 : 177-188, 2003

5 Ryu, C. M., "Tobacco cultivars vary in induction of systemic resistance against Cucumber mosaic virus and growth promotion by Pseudomonas chlororaphis O6 and its gacS mutant" 119 : 383-390, 2007

6 Ryu, C. M., "Tobacco cultivars vary in induction of systemic resistance against Cucumber mosaic virus and growth promotion by Pseudomonas chlororaphis O6 and its gacS mutant" 119 : 383-390, 2007

7 Verhagen, B. W., "The transcriptome of rhizobacteria-induced systemic resistance in Arabidopsis" 17 : 895-908, 2004

8 Verhagen, B. W., "The transcriptome of rhizobacteria- induced systemic resistance in Arabidopsis" 17 : 895-908, 2004

9 Pierpoint, W. S., "The presence of a thaumatin-like protein, a chitinase and a glucanase among the pathogenesis-related proteins of potato (Solanum tuberosum)" 36 : 325-338, 1990

10 Pierpoint, W. S., "The presence of a thaumatin-like protein, a chitinase and a glucanase among the pathogenesis-related proteins of potato (Solanum tuberosum)" 36 : 325-338, 1990

11 Timmusk, S., "The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses" 12 : 951-959, 1999

12 Timmusk, S., "The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses" 12 : 951-959, 1999

13 Kim, Y. C., "The factorial basis for plant health promotion by plant-associated bacteria" 77 : 1548-1555, 2011

14 Kim, Y. C., "The factorial basis for plant health promotion by plant-associated bacteria" 77 : 1548-1555, 2011

15 Glick, B. R., "The enhancement of plant growth by free-living bacteria" 41 : 109-117, 1995

16 Glick, B. R., "The enhancement of plant growth by free-living bacteria" 41 : 109-117, 1995

17 Waller, F., "The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield" 102 : 13386-13391, 2005

18 Waller, F., "The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield" 102 : 13386-13391, 2005

19 Van Loon, L. C., "Systemic resistance induced by rhizosphere bacteria" 36 : 453-483, 1998

20 Van Loon, L. C., "Systemic resistance induced by rhizosphere bacteria" 36 : 453-483, 1998

21 Schardl, C. L., "Symbioses of grasses with seedborne fungal endophytes" 55 : 315-340, 2004

22 Schardl, C. L., "Symbioses of grasses with seedborne fungal endophytes" 55 : 315-340, 2004

23 Fernandez de Caleya, R., "Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro" 23 : 998-1000, 1972

24 Fernandez de Caleya, R., "Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro" 23 : 998-1000, 1972

25 Defago, G., "Pseudomonads as antagonists of soilborne pathogens: modes of action and genetic analysis. In Soil Biochemistry, Vol. 6, ed" Marcel Dekker 249-291, 1990

26 Defago, G., "Pseudomonads as antagonists of soilborne pathogens: modes of action and genetic analysis, In Soil Biochemistry, Vol. 6, ed" Marcel Dekker 249-291, 1990

27 Hong, J. K., "Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis" 22 : 433-448, 2006

28 Hong, J. K., "Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis" 22 : 433-448, 2006

29 Kang, B. R., "Production of indole-3-acetic acid in the plant beneficial strain Pseudomonas chlororaphis O6 is negatively regulated by the global sensor kinase GacS" 52 : 473-476, 2006

30 Kang, B. R., "Production of indole-3-acetic acid in the plant beneficial strain Pseudomonas chlororaphis O6 is negatively regulated by the global sensor kinase GacS" 52 : 473-476, 2006

31 Conrath, U., "Priming in plant-pathogen interactions" 7 : 210-216, 2002

32 Conrath, U., "Priming in plant-pathogen interactions" 7 : 210-216, 2002

33 Mayak, S., "Plant growth-promoting bacteria confer resistance in tomato plants to salt stress" 42 : 565-572, 2004

34 Mayak, S., "Plant growth-promoting bacteria confer resistance in tomato plants to salt stress" 42 : 565-572, 2004

35 Epple, P., "Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum" 9 : 509-520, 1997

36 Epple, P., "Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum" 9 : 509-520, 1997

37 Wang, Y., "Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis" 18 : 385-396, 2005

38 Wang, Y., "Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis" 18 : 385-396, 2005

39 Ramamoorthy, V., "Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases" 20 : 1-11, 2001

40 Ramamoorthy, V., "Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases" 20 : 1-11, 2001

41 Kloepper, J. W., "Induced systemic resistance and promotion of plant growth by Bacillus spp" 94 : 1259-1266, 2004

42 Kloepper, J. W., "Induced systemic resistance and promotion of plant growth by Bacillus spp" 94 : 1259-1266, 2004

43 Spencer, M., "Induced defence in tobacco by Pseudomonas chlororaphis strain O6 involves at least the ethylene pathway" 63 : 27-34, 2003

44 Spencer, M., "Induced defence in tobacco by Pseudomonas chlororaphis strain O6 involves at least the ethylene pathway" 63 : 27-34, 2003

45 조송미, "Induced Systemic Drought and Salt Tolerance by Pseudomonas chlororaphis O6 Root Colonization is Mediated by ABA-independent Stomatal Closure" 한국식물병리학회 28 (28): 202-206, 2012

46 Van Loon, L. C., "Increased plant fitness by rhizobacteria, In Molecular ecotoxicology of plants" Springer-Verlag 177-205, 2004

47 Van Loon, L. C., "Increased plant fitness by rhizobacteria, In Molecular ecotoxicology of plants" Springer-Verlag 177-205, 2004

48 Song Mi Cho, "Identification and Transcriptional Analysis of Priming Genes in Arabidopsis thaliana Induced by Root Colonization with Pseudomonas chlororaphis O6" 한국식물병리학회 27 (27): 272-279, 2011

49 Wittstock, U., "Glucosinolate research in the Arabidopsis era" 7 : 263-270, 2002

50 Wittstock, U., "Glucosinolate research in the Arabidopsis era" 7 : 263-270, 2002

51 Shinozaki, K., "Gene expression and signal transduction in water-stress response" 115 : 327-334, 1997

52 Shinozaki, K., "Gene expression and signal transduction in water-stress response" 115 : 327-334, 1997

53 Kim, M. S., "Gene expression analysis in cucumber leaves primed by root colonization with Pseudomonas chlororaphis O6 upon challenge-inoculation with Corynespora cassiicola" 6 : 105-108, 2004

54 Kim, M. S., "Gene expression analysis in cucumber leaves primed by root colonization with Pseudomonas chlororaphis O6 upon challenge-inoculation with Corynespora cassiicola" 6 : 105-108, 2004

55 Han, S. H., "GacS-dependent production of 2R, 3Rbutanediol by Pseudomonas chlororaphis O6 is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv. tabaci in tobacco. Mol. Plant-Microbe Interact. 19 : 924−930" 19 : 924-930, 2006

56 Han, S. H., "GacS-dependent production of 2R, 3Rbutanediol by Pseudomonas chlororaphis O6 is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv. tabaci in tobacco" 19 : 924-930, 2006

57 Irizarry, R. A., "Exploration, normalization, and summaries of high density oligonucleotide array probe level data" 4 : 249-264, 2003

58 Irizarry, R. A., "Exploration, normalization, and summaries of high density oligonucleotide array probe level data" 4 : 249-264, 2003

59 Kim, Y. C., "Enhancement of plant drought tolerance by microbes, In Plant responses to drought stress: From Morphological to molecular feature" Springer-Verlag 2012

60 Kim, Y. C., "Enhancement of plant drought tolerance by microbes, In Plant responses to drought stress: From Morphological to molecular feature" Springer-Verlag 2012

61 Kloepper, J. W., "Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria" 286 : 885-886, 1980

62 Kloepper, J. W., "Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria" 286 : 885-886, 1980

63 Epple, P., "Differential induction of the Arabidopsis thaliana Thi2.1 gene by Fusarium oxysporum f. sp. matthiolae" 11 : 523-239, 1998

64 Epple, P., "Differential induction of the Arabidopsis thaliana Thi2.1 gene by Fusarium oxysporum f. sp. matthiolae" 11 : 523-239, 1998

65 Edens, L., "Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli" 18 : 1-12, 1982

66 Edens, L., "Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli" 18 : 1-12, 1982

67 Hontzeas, N., "Changes in gene expression in canola roots induced by ACC-deaminse-containing plant-growth-promoting bacteria" 17 : 865-871, 2004

68 Hontzeas, N., "Changes in gene expression in canola roots induced by ACC-deaminse-containing plant-growth-promoting bacteria" 17 : 865-871, 2004

69 Hong, J. K., "Activation of pepper basic PR-1 gene promoter during defense signaling to pathogen, abiotic and environmental stresses" 356 : 169-180, 2005

70 Hong, J. K., "Activation of pepper basic PR-1 gene promoter during defense signaling to pathogen, abiotic and environmental stresses" 356 : 169-180, 2005

71 Choi, H. I., "ABFs, a family of ABA-responsive element binding factors" 275 : 1723-1730, 2000

72 Choi, H. I., "ABFs, a family of ABA-responsive element binding factors" 275 : 1723-1730, 2000

73 Cornelissen, B. J. C., "A tobacco mosaic virus-induced protein is homologous to the sweet-tasting protein thaumatin" 321 : 531-532, 1986

74 Cornelissen, B. J. C., "A tobacco mosaic virus-induced protein is homologous to the sweet-tasting protein thaumatin" 321 : 531-532, 1986

75 Vigers, J. A., "A new family of plant antifungal proteins" 4 : 315-323, 1992

76 Vigers, J. A., "A new family of plant antifungal proteins" 4 : 315-323, 1992

77 Glick, B. R., "A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria" 190 : 63-68, 1998

78 Glick, B. R., "A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria" 190 : 63-68, 1998

79 Cho, S. M., "2R,3R-butanediol, a bacterial volatile produced by Pseudomonas chlororaphis O6, is involved in induction of systemic tolerance to drought in Arabidopsis thaliana" 21 : 1067-1075, 2008

80 Cho, S. M., "2R,3R-butanediol, a bacterial volatile produced by Pseudomonas chlororaphis O6, is involved in induction of systemic tolerance to drought in Arabidopsis thaliana" 21 : 1067-1075, 2008