현사시나무에서 SENESCENCE 1 유전자의 분리와 발현특성 구명 = Isolation and Characterization of a Putative SENESCENCE 1 Gene from Poplar (Populus alba ⨯ P. glandulosa)

국문 초록 (Abstract)

본 연구에서는 식물의 노쇠와 관련된 SENESCENCE 1 (SEN1)유전자를 현사시나무에서 분리하고, 여러 가지 조건에서 발현특성을 분석하였다. 현사시나무의 SEN1 유전자(PagSEN1)는243개의 아미노산으로 이루어져 있고, 한 개의 rhodanese domain을 가지고 있다. Southern blot 분석 결과 PagSEN1 유전자는현사시나무에서 2 copy 정도가 존재하는 것으로 나타났다. 조직 특이적 발현양상을 분석한 결과 PagSEN1 유전자는 성숙잎에서 가장 높게 발현하고, 뿌리에서 가장 낮게 발현하는 것으로나타났다. 또한 mannitol과 염 스트레스 처리에 의해 300배 이상 발현이 증가한 반면에 저온 스트레스에는 반응하지 않았다.
식물 호르몬 처리에서는 ABA와 JA 처리 10시간 후에 발현이 3.5배와 2.4배 이상 증가하는 것으로 나타났다. 따라서 PagSEN1유전자는 건조와 염 스트레스에 반응하며, 식물의 자연적 노쇠과정 뿐 아니라 스트레스와 같은 환경변화에 의해 유발되는 노쇠과정에도 관여하는 것으로 판단된다.

다국어 초록 (Multilingual Abstract)

Plant senescence is one of the survival strategies to use limited nutrients efficiently during growth, development and adaptation. In this study, we isolated a gene (PagSEN1) homologous to SENESCENCE 1 from Populus alba ⨯ P.
glandulosa. The PagSEN1 gene encodes a putative protein consisting of 243 amino acids containing a rhodanese domain.
Southern blot analysis suggested that two copies of the PagSEN1 gene are present in the poplar genome. We characterized its transcriptional expression under various conditions mimicking senescence and environmental stresses. The PagSEN1 was expressed most strongly in mature leaves but most weakly in roots. The gene was significantly up-regulated by treatments with mannitol, NaCl, ABA and JA, but not by cold, SA and GA 3 . These results indicate that PagSEN1 is involved in senescence response induced by environmental stresses.

참고문헌 (Reference)

1 Kim, Y.H., "Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance" 9 : 334-347, 2011

2 Agarwal, P.K, "Transcription factors in plants and ABA dependent and independent abiotic stress signalling" 54 : 201-212, 2010

3 van der Graaff, E., "Transcription analysis of Arabidopsis membrane transporters and hormone pathways during developmental and induced leaf senescence" 141 : 776-792, 2006

4 Schenk, P.M., "The SEN1 gene of Arabidopsis is regulated by signals that link plant defence responses and senescence" 43 : 997-1005, 2005

5 Tuskan, G.A., "The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray)" 313 : 1596-1604, 2006

6 Gepstein, S., "Strategies to ameliorate abiotic stress-induced plant senescence" 82 : 623-633, 2013

7 Thomas, H., "Senescence, ageing and death of the whole plant" 197 : 696-711, 2013

8 Fuqiang, C., "Regulation of ABA dependent wound induced spreading cell death by MYB 108" 200 : 634-640, 2013

9 Rohde, A., "Plant dormancy in the perennial context" 12 : 217-223, 2007

10 Lee, D.W., "Pigment dynamics and autumn leaf senescence in a New England deciduous forest, eastern USA" 18 : 677-694, 2003

11 Kramell, R., "Occurrence and identification of jasmonic acid and its amino acid conjugates induced by osmotic stress in barley leaf tissue" 14 : 29-36, 1995

12 Himelblau, E., "Nutrients mobilized from leaves of Arabidopsis thalinana during leaf senescence" 158 : 1317-1323, 2001

13 Yang, S.H., "Ntdin, a tobacco senescence-associated gene, is involved in molybdenum cofactor biosynthesis" 44 : 1037-1044, 2003

14 Hörtensteiner, S., "Nitrogen metabolism and remobilization during senescence" 53 : 927-937, 2002

15 Shinozaki, K., "Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways" 3 : 217-223, 2000

16 Xiong, L., "Molecular and genetic aspects of plant responses to osmotic stress" 25 : 131-139, 2002

17 Ueda, J., "Isolation and identification of a senescence-promoting substance from wormwood (Artemisia absinthium L.)" 66 : 246-249, 1980

18 이효신, "Isolation and Characterization of Homeodomain-Leucine Zipper III Protein 1 Gene in Poplar" 한국육종학회 1 (1): 236-244, 2013

19 Lee, H., "Generation and analysis of expressed sequence tags from poplar (Populus alba × P. tremula var. glandulosa) suspension cells" 169 : 1118-1124, 2005

20 Azumi, Y., "Evidence of a senescenceassociated gene induced by darkness" 95 : 577-583, 1991

21 Cipollone, R., "Enzymatic detoxification of cyanide: clues from Pseudomonas aeruginosa Rhodanese" 15 : 199-211, 2008

22 Southern, E.M., "Detection of specific sequences among DNA fragments" 98 : 503-517, 1975

23 Jukanti, A.K., "Comparative transcriptome profiling of near-isogenic barley (Hordeum vulgare) lines differing in the allelic state of a major grain protein content locus identifies genes with possible roles in leaf senescence and nitrogen reallocation" 177 : 333-349, 2008

24 Buchanan-Wollaston, V., "Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis" 42 : 567-585, 2005

25 AGI, "Analysis of the genome sequence of the flowering plant Arabidopsis thaliana" 408 : 796-815, 2000

26 Oh, S.A., "A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence" 30 : 739-754, 1996

27 Pfaffl, M.W., "A new mathematical model for relative quantification in real-time RT-PCR" 29 : 2002-2007, 2001

28 Keskitalo, J., "A cellular timetable of autumn senescence" 139 : 1635-1648, 2005