<B>ABSTRACT</B><P><I>Orientia tsutsugamushi</I>, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. We observed the localiza...
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https://www.riss.kr/link?id=A107529011
2006
-
SCI,SCIE,SCOPUS
학술저널
4246-4253(8쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<B>ABSTRACT</B><P><I>Orientia tsutsugamushi</I>, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. We observed the localiza...
<B>ABSTRACT</B><P><I>Orientia tsutsugamushi</I>, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. We observed the localization of <I>O. tsutsugamushi</I> with clathrin or adaptor protein 2 within 30 min after the infection of nonprofessional phagocytes. We have further confirmed that the infectivity of <I>O. tsutsugamushi</I> is significantly reduced by drugs that block clathrin-mediated endocytosis but not by filipin III, an inhibitor that blocks caveola-mediated endocytosis. In the present study, with a confocal microscope, <I>O. tsutsugamushi</I> was sequentially colocalized with the early and late endosomal markers EEA1 and LAMP2, respectively, within 1 h after infection. The colocalization of <I>O. tsutsugamushi</I> organisms with EEA1 and LAMP2 gradually disappeared until 2 h postinfection, and then free <I>O. tsutsugamushi</I> organisms were found in the cytoplasm. When the acidification of endocytic vesicles was blocked by treating the cells with NH4Cl or bafilomycin A, the escape of <I>O. tsutsugamushi</I> organisms from the endocytic pathway was severely impaired, and the infectivity of <I>O. tsutsugamushi</I> was drastically reduced. To our knowledge, this is the first report that the invasion of <I>O. tsutsugamushi</I> is dependent on the clathrin-dependent endocytic pathway and the acidification process of the endocytic vesicles in nonprofessional phagocytes.</P>