느타리 수확후배지로부터 분리된 고온성 Bacillus licheniformis YJ09의 특성

다국어 초록 (Multilingual Abstract)

In order to isolate thermophilic bacteria with high activity of CMCase and xylanase, spent mushroom substrates was collected from an oyster mushroom cultivation farm in Jinju, Gyeongnam, Korea. Among the isolates, one strain designated as YJ09 was selected by agar diffusion method. The isolate YJ09 was identified as a member of Bacillus licheniformis based on biochemical characteristics using Bacillus ID kit and MicroLog system. Comparative 16S rDNA sequence analysis showed that isolate YJ09 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus licheniformis with sequence similarity of 98.9%. Based on its physiological properties, biochemical characteristics and phylogenetic distinctiveness, the isolate YJ09 was classified as Bacillus licheniformis. The CMCase and xylanase activity of B. licheniformis YJ09 was slightly increased corresponding to the bacterial population from exponential phase to stationary phase in the growth curve of B. licheniformis YJ09.

목차 (Table of Contents)

• ABSTRACT
• 부숙촉진 미생물의 분리
• 분리균의 동정
• 분리균의 생육과 효소 생성능
• 적요
• 참고문헌

참고문헌 (Reference)

1 김지연, "알칼리성 Cellulase를 생산하는 호알칼리성 Bacillus sp. HSH-810의 분리 및 효소 특성" 한국미생물학회 40 (40): 139-146, 2004

2 신평균, "부숙촉진 세균 Bacillus sp. SJ21 균주의 cellulase와 xylanase 활성" 한국토양비료학회 44 (44): 836-840, 2011

3 LEE, JUN-HO, "Xylanase Production by Bacillus sp. A-6 Isolated from Rice Bran" 한국미생물·생명공학회 16 (16): 1856-1861, 2006

4 Miller GL, "Measurement of carboxymethyl cellulase activity" 2 : 127-132, 1960

5 Kim DJ, "Isolation of a Thermophilic Bacillus sp. producing the thermostable cellulase-free xylanase, and properties of the enzyme" 23 : 304-310, 1995

6 Schallmey M, "Developments in the use of Bacillus species for industrial production" 50 : 1-17, 2004

7 Seki T, "Deoxyribonucleic acid homology and taxonomy of the genus Bacillus" 28 : 182-189, 1978

8 Schafer T, "Biopolymers" Wiley VCH Editor 377-437, 2005

9 Williams BC, "An initial assessment of spent mushroom compost as apotential energy feedstock" 79 : 227-230, 2001