Dual wavelength Nonaqueous Capillary Electrophoresis for High throughput Screening of Synthetic Organic Dyes

다국어 초록 (Multilingual Abstract)

A novel dual-wavelength nonaqueous capillary electrophoresis (DW-NACE) with a laser-induced fluorescence detector was developed for the high-throughput screening of various synthetic organic dyes.
Different synthetic organic dyes were simultaneously detected and analyzed by dual-wavelength excitation light sources [e.g., 543 nm for crystal violet (CV), methyl violet B (MVB), methyl violet B base (MBB), rhodamine 6G (R6G), and rhodamine B base (RBB); 635 nm for nile blue A (NBA) and methylene blue (MB)]. The dual-laser detection technique has been shown to be an effective strategy for simultaneous detection with higher efficiencies.
Synthetic organic dyes experiment can be extended into wide range by increasing the solublity of the synthetic organic dyes in nonaqueous solvent. Moreover, nonaqueous solvent can hold a higher electric field than aqueous solvent because of reduction in Joule heating. Using nonaqueous solvent, the CE technique was faster, more selective, and sensitive than aqueous solvent.
The synthetic organic dyes were analyzed within 15 min with excellent separation performance under an electric field of 500 V/cm at DW-NACE. The combination of a nonaqueous solvent and a dual-wavelength detection of capillary electrophoresis system enable the simultaneous analysis of various organic dyes with rapid detection, high detection sensitivity, and excellent reproducibility.

국문 초록 (Abstract)

새로운 레이저-유발 형광 검출기가 (laser-induced fluorescence detector) 장착 된 이중-파장 비-수용성 모세관 전기영동 (dual-wavelength nonaqueous capillary electrophoresis, DW-NACE)은 다양한 합성 유기염료를 고속대량스크리닝을 하기 위해 개발되었다.
다른 들뜸 파장은 갖는 합성 유기염료는 이중-파장 들뜸 광원에 의해 동시에 검출 및 분석된다 [예, 543 nm for crystal violet (CV), methyl violet B (MVB), methyl violet B base (MBB), rhodamine 6G (R6G), and rhodamine B base (RBB); 635 nm for nile blue A (NBA) and methylene blue (MB)]. 이중-레이저 검출 기술은 높은 효율을 가진 동시 검출을 할 수 있는 효과적인 장점을 보여준다.
유기 합성 염료 용해도는 비-수용성 용매에서 증가하므로 유기 합성 염료는 다양한 범위에서 실험이 가능하다. 더욱이 비-수용성 용매는 주울열의 (Joule heating) 발생이 감소하므로 높은 전압을 걸어줄 수 있다. 비-수용성 용매를 사용하면, 모세관 전기영동 기술은 수용성 용매보다 빠르고, 선택성이 있고, 감도가 높다.
이중-파장 비-수용성 모세관 전기영동은 500 V/cm의 전기장에서 높은 분리 효율을 가지며 15분 이내에 합성 유기 염료가 분석된다. 모세관 전기영동에 비-수용성 용매와 이중-파장 검출이 혼합 된 시스템은 빠른 검출과 높은 검출 감도 및 높은 정밀성을 가지며 다양한 유기염료를 동시 분석할 수 있음을 입증하였다.

목차 (Table of Contents)

• CONTENTS
• ABSTRACT •••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• i
• CHAPTER І. General Introduction
• I-1. Capillary Electrophoresis ••••••••••••••••••••••••••••• 1
• I-2. Nonaqueous Capillary Electrophoresis ••••••••••••••• 13
• I-3. References •••••••••••••••••••••••••••••••••••••••••••••••• 21
• CHAPTER II. Dual-wavelength Nonaqueous Capillary Electrophoresis for High-throughput Screening of Synthetic Organic Dyes
• Abstract •••••••••••••••••••••••••••••••••••••••••••••••••••••••• 25
• Introduction •••••••••••••••••••••••••••••••••••••••••••••••••••• 27
• Experimental •••••••••••••••••••••••••••••••••••••••••••••••••• 29
• Results and Discussions ••••••••••••••••••••••••••••••••••••• 41
• Conclusion ••••••••••••••••••••••••••••••••••••••••••••••••••••• 58
• Acknowledgements ••••••••••••••••••••••••••••••••••••••••••• 59
• References ••••••••••••••••••••••••••••••••••••••••••••••••••••• 59
• ABSTRACT IN KOREAN ••••••••••••••••••••••••••••••••••••••••••• 63
• APPENDIX I ••••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 65
• APPENDIX II •••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 66
• APPENDIX II •••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 73
• ACKNOWLEDGEMENT •••••••••••••••••••••••••••••••••••••••••••• 75