Pga gene incoding the penicillin G acylase(EC 3,5,1,11, benzylpenicillin acylase, PGA) was characterized by E. coli BL(DE3) harboring pga gene. PGA was a heterodimeric form consisted 65kD and 24kD subunit with SDS-PAGE and it was localized in cytoplas...
Pga gene incoding the penicillin G acylase(EC 3,5,1,11, benzylpenicillin acylase, PGA) was characterized by E. coli BL(DE3) harboring pga gene. PGA was a heterodimeric form consisted 65kD and 24kD subunit with SDS-PAGE and it was localized in cytoplasm. When E. coli BL(DE3) was cultivated at 37℃ and then shifted the temperature with 33℃ and 29℃, the cell growth was higher at 37℃ than 33℃ and 29℃, but the production of enzyme more increased at lower temperature than higher temperature. When the strain was cultivated at 37℃ and shifted gradually untill 29℃ during 40 hours, the maximum activity of the enzyme was 2.45 U/㎖