Effect of the Extract of Styphnolobium japonicum Fruit on the Secretion of Tissue-Damaging Mediators From Macrophages and Keratinocytes Following Exposure to External Triggers

다국어 초록 (Multilingual Abstract)

Styphnolobium japonicum belongs to the Fabaceae family. This plant, particularly its fruit, is widely used in traditional medicine to treat various disorders. Lately, there has been an increased interest in using the salt-out approach for extracting active substances from plants, leading to improved yields. The purpose of this study was to prepare an extract from the S. japonicum fruit using the salt-out method (SJFSOE) and explore its benefits by examining its effects on the secretion of nitic oxide (NO) from lipopolysaccharide (LPS)-treated Raw264.7 cells (murine macrophage cells) and release of matrix metalloproteases (MMPs) from ultraviolet B (UVB)-irradiated CCD-986sk cells (normal human skin fibroblast cells). Our findings show that SJFSOE contains mainly sophoricoside (877.3 mg/g of the extract), that is over twenty times that obtained by a method using ethanol, and significantly suppressed both LPS-induced secretion of NO and UVB-stimulated release of the pro-MMP-1 and MMP-2 from the respective cells. The results imply that SJFSOE has the potential to offer benefits, especially in conditions characterized by inflammation.