Isolation and Characterization of a Novel Heterotrophic Nitrifying and Aerobic Denitrifying Bacterium Pseudomonas stutzeri KTB for Bioremediation of Wastewater

다국어 초록 (Multilingual Abstract)

A novel heterotrophic nitrifying and aerobicdenitrifying bacterium, KTB, was isolated from activatedsludge flocci collected from a biological aerated filteraccording to the modified Takaya method and identified asPseudomonas stutzeri by 16S rDNA gene sequence analysis.
When shaking-cultured in the presence of 4.331 mmol/L ofnitrate, 4.511 mmol/L of nitrite and 4.438 mmol/L ofammonium, the strain grew fast, with μmax being 0.42, 0.45,and 0.56/h, and displayed high nitrogen removal efficiency,with nitrogen removal rate being 0.239, 0.362, and 0.361mmol/L/h and nitrogen removal ratio being 99.1, 100.0,and 100.0% in 18 h, respectively. The removal mainlyoccurred in the logarithmic phase. Nitrite accumulation didnot affect denitrification performance. Nitrate concentrationwas below the detectable limit during the whole growthcycle when ammonium was used as sole nitrogen source.
It tolerated high DO level and exhibited excellent aggregationability. A possible pathway involved in the nitrogenremoval process, which demonstrated a full nitrificationand denitrification route, was speculated. The strain mightbe a great candidate for biological removal of nitrogencompounds from wastewater.

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