Changes in the Content of Total Phenolics and Inorganic Peroxides in the Rice Seedings under Low Temperature = 저온처리한 벼 유식물의 총 페놀화합물 및 무기 과산화물 함략의 변화

다국어 초록 (Multilingual Abstract)

To investigate the effects of low temperature on the chilling-sensitive plants, accumulations of some kinds of toxic materials in the rice seedlings under low temperature leading to chilling injury were investigated. Activities of some enzymes responsible for their metabolism were also determined during chilling treatment. Total phenolics were gradually increased during the treatmetn period of both 15℃ and 4℃ in a similar pattern. Activities of PAL, the key enzyme of shikimate pathway, were also changed in a manner of about two-fold increase under low temperature. The content of inorganic peroxides showed an initial increase of 67% and a subsequent decline at 15℃, while it showed a maximal increase of 73% after 5 day duration at 4℃. The changes in the amount of inorganic peroxides present in the rice seedlings under chilling temperature were thought to be related to the affected activity of catalase, SOD and peroxidase. Catalase was decreased upon chilling and SOD activity showed an initial increase and later decrease relative to control plants. Peroxidase, however, was decreased relative to control plants, while the activity was shown to increase gradually with the progress of chilling. Therefore, it coulud be suggested that the amount of total phenolics and inorganic peroxides present in the rice seedlings under low temperature were mostly dependent on the activities of the enzymes responsible for their metabolism affected by chilling stress.

목차 (Table of Contents)

• INTRODUCTION
• MATERIALS AND METHODS
• Plant material
• Chilling treatment
• Determination of total phenolics and inorganic peroxides
• Standard crude enzyme extraction
• Enzyme assays
• RESULTS AND DISCUSSION
• Changes in the amount of total phenolics
• The activities of PAL
• Changes in the amount of inorganic peroxides
• The activities of catalase
• The activities of SOD
• The activities of peroxidase