Laurencia okamurai Extract Containing Laurinterol Induces Apoptosis in Melanoma Cells

다국어 초록 (Multilingual Abstract)

Laurinterol is a marine sesquiterpene that has been known to have antimicrobial activity. The purpose of thisstudy is to investigate the effect of Laurencia okamuraiextract containing laurinterol (LOEL) on induction of apoptosis inmelanoma cells (B16F1). Anticancer activity of LOEL against melanoma cells was shown in a dose-dependent manner by the1-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. It was for the first time found that LOEL exhibitedan excellent effect on the induction of apoptosis as determined by DNA fragmentation, terminal deoxynucleotidyl transferase-mediated dUTP in situnick-end labeling assay, cell cycle analysis, and measurement of activities of several caspases inmelanoma cells. It was also demonstrated that transcriptional activation of p53, a tumor suppressor gene, and activation ofp21 promoter by LOEL were involved in the induction of apoptosis by reporter gene assay. In particular, western blot analy-sis confirmed that LOEL above 5 .g/mL significantly increased the expression level of phospho-p53, the active form. Theseresults indicate that LOEL can induce apoptosis through a p53-dependent pathway in melanoma cells.

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