능성어(Epinephelus septemfasciatus) 치어의 생존율과 혈액학적 특성 변화에 미치는 암모니아 노출의 영향 = Effects of Ammonia Exposure on Survival Rate and Hematological Characteristics Changes in Juveniles of Sevenband Grouper, Epinephelus septemfasciatus

다국어 초록 (Multilingual Abstract)

We investigated that changes of survival rate and hematological factors in sevenband grouper (Epinephelus septemfasciatus), exposed to ammonia. First, the fish was exposed and was observed survival rate. We observed the survival rate of the fish when exposed at each ammonia concentrations (0, 0.35, 0.4, 0.5, 0.8 mg/L, NH₃) for 96 hours. The 96-h LC₅₀ (lethal ambient concentration for 50% of the population) of NH₄⁺ and un-ionized ammonia (NH₃) for sevenband grouper were 26.7 and 0.6 mg/L, respectively. Secondly, we exposed the fish to 96-h LC₅₀ ammonia concentration (0.6 mg/L) for 96 hours, then sampled the blood of groupers for analysis of cortisol, glucose, NH₃, GOT, GPT, total protein (TP), electrolytes (Na⁺, Cl_-) and osmolality in plasma. The plasma cortisol, glucose, GOT and GPT were increased with increasing of elapsed time and ammonia concentration. In addition, the NH₃ and total protein in plasma were significantly increased to 12 hours exposed to ammonia, and then decreased. But, the values were significantly higher than the control. The plasma electrolytes (Na⁺, Cl^-) and osmolality were decreased with increasing of elasped time. The results in the present study suggest that the continuous exposure of ammonia to juveniles of sevenband grouper may be a stressor and negative influence.

참고문헌 (Reference)

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