Effect of Synbiotic Supplementation Containing Cornus fructus and Lactobacillus reuteri on Cognitive Impairment in DSS-Induced Colitis Mice

다국어 초록 (Multilingual Abstract)

This study was conducted to assess the synbiotics interaction between the Corni fructus and Lactobacillus reuteri in a dextran sulfate sodium (DSS)-induced colitis and cognitive dysfunction in C57BL/6 mice. Mice were treated with Corni fructus (PRE; prebiotics), Lactobacillus reuteri (PRO; probiotics), and synbiotics (SYN; combination of Lactobacillus reuteri and Corni fructus) for 3 weeks. Consumption of PRE, PRO and SYN ameliorated body weight loss and colon-length shortening. Those treatments improved intestinal permeability by decreasing of in serum and increasing of tight junction protein expression in colonic tissue. Those also decreased the expression of inflammatory cytokine, and the myeloperoxidase activity. Especially, SYN significantly increased the content of short chain fatty acids in colitis mice fecal compared to PRE and PRO. Furthermore, PRE, PRO and SYN improved behavioral dysfunction by evaluating the Y-maze and passive avoidance tests. SYN significantly protected the antioxidant damages and cholinergic dysfunction, and regulated protein expression of acetylcholinesterase and choline acetyltransferase compared with PRE and PRO.