Characters of Extracellular β-Lactamase Obtained from a Strain of Streptomyces sp. = 방선균의 일주가 생성하는 균체외 β- Lactamase의 특성

다국어 초록 (Multilingual Abstract)

A strain of Streptomyces sp. isolated from soil was found to produce extra-cellular β-lactamase associated partially to the cell growth. The β-lactamase was purified from the culture supernatant through ammonium sulfate fractionation, ion-exchange chromatographies and gel filtration. The final purification fold and recovery yield were 57 and 6.2%, respectively. Molecular weight of the β-lactamase was estimated to be about 67,000 by SDS-polyacrylamide gel electrophoresis. The optimal reaction condition was at pH 7∼8 and at 35∼45℃. The K_m and V_max values of the enzyme for penicillin G were estimated to be 3 mM and 1.3×10 exp(3) uM/min/㎎ protein, respectively. The purified β-lactamase was classified to the class A enzyme hydrolyzing only penicillin.