Dextromethorphan 이 마우스의 비장세포 증식능과 cytokine 생성능에 미치는 영향 = Effects of Dextromethorphan on the proliferation and cytokines production of mouse splenocytes

다국어 초록 (Multilingual Abstract)

Dextromethorphan hydrobromide (DXM) has been widely used as a nonopioid antitussive drug and is a psychotropic drug since 2003 in our country. This study was performed to investigate the immunotoxicity of DXM by assay of splenocytes proliferation (SP) and induction of cytokine (IFN-γ, IL-4) in vitro. When mouse splenocytes were exposed to various concentration of DXM (0.001, 0.01, 0.1, 1, 10, 100μM) and cultured with T cell mitogen (Con A) or B cell mitogen (LPS), SP to Con A and LPS were significantly decreased at high concentration (100μM) when compared with controls. When splenocytes were cultured with DXM in the presence of Con A, levels of IFN-γ in culture supernatant were slightly decreased at low concentration, and significantly decreased at high concentration (100μM). DXM also suppressed IL-4 secretion significantly at 100μM, however, increased the production of IL-4 at lower concentration when compared with control group. This study provides the substantial evidence on DXM-induced alternation in cell-mediated and humoral immunity.