(The) study of the relationship between the change of PTEN and antioxidant enzyme by antioxidant coenzymeQ10

다국어 초록 (Multilingual Abstract)

Coenzyme Q10 is called to vitamin Q10, ubiquinone or ubidecarenone. This is a component of the mitochondrial electron transport chain and various cellular membranes [1]. It acts as an important in vivo antioxidant. Some Data suggest that consumption of CoQ10 can be protective against the development of prostate cancer. The CoQ10 may regulate proteins that control cellular division, such as the tumor suppressor PTEN(Phosphatase and tensin homologue). But the detailed mechanism of antioxidant effect of coenzyme Q10 on the cell functions has not been examined.
In the present study, we investigated the relationship between the changes of PTEN and antioxidant enzyme by CoQ10 in prostate cancer cell (DU145). We explained thing that CoQ10 had antioxidant effect regarding oxygen free radical generation occurrend when an energy becomes a generation in mitochondria of a DU145 cell. PTEN protein level increased for the correspondence to CoQ10. CoQ10 increased PTEN, and we found it to affect to expression of antioxidant enzymes such as MnSOD, CuZnSOD and Catalase. This suggests thing at DU145 cell that we caused antioxidant enzymes increase by overexpression of PTEN by increase of concentration of CoQ10. We show that a cell decreases through cell viability measurement according to the concentration of CoQ10 at DU145 cell.
These findings suggest an essential role for PTEN in prostate cancer cells against oxidative stress. It suggests that dietary changes may be adjunctive to traditional preventive and therapeutic strategies aginst prostate cancer.

목차 (Table of Contents)

• Abstract 1
• Introduction 2
• Material and Method 5
• Results 8
• Discussion 11
• Figures 14
• Reference 20
• 국문요지 25