Effects of Genistein and Ipriflavone on the Proliferation and Activity of Bone Cells

다국어 초록 (Multilingual Abstract)

Osteoporosis is a metabolic bone disease which characteristically occurs when bone resorption exceeds bone formation, thus resulting in an overall reduction of total skeletal mass. The potential importance of phytoestrogens, especially those steroid-like molecules derived from soybeans is now being actively investigated. The present study was undertaken to determine the possible cellular mechanism of actions of genistein and ipriflavone which was known to modulate bone metabolism. The number of ROS17/2.8 and HOS cells increased significantly when treated with lower concentration of genistein. However, higher concentration of genistein significantly inhibited the proliferation of osteoblastic cells. In contrast, ipriflavone dose-dependently decreased the number of osteoblastic cells. Viability of osteoblastic cells was increased significantly at lower concentration, and decreased at higher concentration of genistein treatment. Ipriflavone decreased the viability of ROS17/2.8 and HOS cells. Genistein increased the ALP activity of osteoblastic cells after 48 hours treatment. Ipriflavone decreased the ALP activity of osteoblastic cells. TRAP(+) multinucleated cells were generated when RAW 264.7 cells were cultured in the presence of RANKL and TGF-β. Genistein decreased the number of TRAP(+)-multinucleated cells at 10^-9 - 10^-7M concentrations, however, higher concentration of genistein (10^-5M) sigificantly increased the number of TRAP(+)-multinucleated cells. Ipriflavone significantly increased the number of TRAP(+)-multinucleated cells. High dose of ipriflavone (10^-5M) showed the cytotoxicity on the RAW264.7 cells. Thses results indicate that genistein has the biphasic effect on bone metabolism, while ipriflavone inhibit proliferation and activity of osteoblasts and stimulate formation of osteoclasts.