Antinociception Effect and Mechanisms of Campanula Punctata Extract in the Mouse

다국어 초록 (Multilingual Abstract)

In the present study, the antinociceptive profiles of Campanula punctata extract were examined in ICR mice. The Campanula punctata contain a large dose of saponin. Campanula punctata extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, Campanula punctata extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.)injection of substance P (0.7 μg) was diminished by Campanula punctata extract. Intraperitoneal (i.p.)pretreatment with yohimbine (α2-adrenergic receptor antagonist) attenuated antinociceptive effect induced by Campanula punctata extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by Campanula punctata extract in the writhing test. Our results suggest that Campanula punctata extract shows an antinociceptive property in various pain models. Furthermore, this antinociceptive effect of Campanula punctata extract may be mediated by α2-adrenergic receptor, but not opioidergic and serotonergic receptors.

참고문헌 (Reference)

1 Vyklicky L, "The techniques for the study of pain in animals. In Adv Pain Res Ther. Vol. 3." Raven 727-745, 1979

2 Grumbach L, "The prediction of analgesic activity in man by animal testing. In Pain" Little Brown and Co 163-182, 1966

3 Eddy NB, "Synthetic analgesics. II. Dithienylbutenyl- and dithienylbutylamines" 107 : 385-393, 1953

4 Kathleen N, "Sunset Western Garden Book. 6th ed" Sunset Publishing Corporation 606-607, 1995

5 Jensen TS, "Spinal monoamine and opiate systems partly mediate the antinociceptive effects produced by glutamate at brainstem sites" 321 : 287-297, 1984

6 Shin CY, "Platycodin D and D3 increase airway mucin release in vivo and in vitro in rats and hamsters" 68 : 221-225, 2002

7 Chapman CR, "Pain measurement: an overview" 22 : 1-31, 1985

8 Yaksh TL, "Multiple opioid receptor systems in brain and spinal cord: Part I" 1 : 171-199, 1984

9 Hylden JL, "Intrathecal substance P elicits a caudally- directed biting and scratching behavior in mice" 217 : 212-215, 1981

10 Hylden JL, "Intrathecal morphine in mice: a new technique" 67 : 313-316, 1980

11 Schmauss C, "In vivo studies on spinal opiate receptor systems mediating antinociception. II. Pharmacological profiles suggesting a differential association of mu, delta and kappa receptors with visceral chemical and cutaneous thermal stimuli in the rat" 228 : 1-12, 1984

12 Cumberbatch MJ, "Exposure of rat spinal neurons to NMDA, AMPA and kainate produces only short-term enhancements of responses to noxious and non-noxious stimuli" 181 : 98-102, 1994

13 Suh HW, "Effect of histamine receptor antagonists injected intrathecally on antinociception induced by opioids administered intracerebroventricularly in the mouse" 33 : 121-129, 1999

14 Yaksh TL, "Direct evidence that spinal serotonin and noradrenaline terminals mediate the spinal antinpciceptive effects of morphine in the periaqueductal gray" 160 : 180-185, 1979

15 Suh HW, "Differntial effects of adenosine receptor antagonist injected intrathecally on antinociception induced by morphine and beta-endorphin administered intracerebroventricularly in the mouse" 31 : 339-344, 1997

16 Wigdor S, "Central and systemic morphine-induced antinociception in mice: contribution of descending serotonergic and noradrenergic pathways" 242 : 90-95, 1987

17 Suh HW, "Antinociceptive mechanisms of dipsacus saponin C administered intracerebroventricularly in the mouse" 27 : 1167-1172, 1996

18 Soo-Hyun Park, "Antinociceptive Profiles and Mechanisms of Orally Administered Vanillin in the Mice" 대한약학회 32 (32): 1643-1649, 2009

19 Koster R, "Acetic acid for analgesic screening" 18 : 4-12, 1959

20 Choi SS, "A tinociceptive mechanisms of orally administered decursinol in the mouse" 13 : 471-485, 2003

21 D’Amour FE, "A method for determining loss of pain sensation" 72 : 74-79, 1941