Inhibitory effects of sesamin on human osteoclastogenesis

다국어 초록 (Multilingual Abstract)

The promotional nature of sesamin on humanosteoblast differentiation has been proven. Here, the effectof sesamin on human osteoclasts was investigated in termsof differentiation and function in M-CSF and RANKLinduced human PBMCs. Sesamin treatment significantlydecreased the number of differentiated osteoclastsobserved by TRAP staining; however, sesamin inhibitiondid not result from the alteration of precursor cell proliferation.
Sesamin did not decrease NFATc1 gene expression,which opposed the decreasing trend of CathK andTRAP expression. DC-STAMP, but not Atp6v0d2, alsosignificantly decreased in the presence of 14 lM sesamin.
Expressions of CCR2b and CCR4 as chemokine receptorswere significantly down-regulated. Sesamin might mediatethe inhibition of human osteoclast differentiation, therecruitment of precursor cells and F-actin formation.
Decrease in the area of the resorption pits and the collagenreleased from the bone slices under sesamin treatmentemphasized the inhibitory effects on both the differentiationand function of osteoclasts. Sesamin is a promisingphytochemical agent inhibiting osteoclast differentiationand function.

참고문헌 (Reference)

1 Spencer GJ, "Wnt signalling in osteoblasts regulates expression of the receptor activator of NFkappaB ligand and inhibits osteoclastogenesis in vitro" 119 (119): 1283-1296, 2006

2 Wanachewin O, "The promoting effects of sesamin on osteoblast differentiation of human mesenchymal stem cells" 14 : 395-406, 2015

3 Lee SE, "The phosphatidylinositol 3-kinase, p38, and extracellular signal-regulated kinase pathways are involved in osteoclast differentiation" 30 (30): 71-77, 2002

4 Asagiri M, "The molecular understanding of osteoclast differentiation" 40 (40): 251-264, 2007

5 Woessner JF Jr, "The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid" 93 : 440-447, 1961

6 Lee ZH, "Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts" 305 (305): 211-214, 2003

7 Hsieh C-C, "Sesamin suppresses macrophage-derived chemokine expression in human monocytes via epigenetic regulation" 5 (5): 2494-2500, 2014

8 Orawan Wanachewin, "Sesamin stimulates osteoblast differentiation through p38 and ERK1/2 MAPK signaling pathways" Springer Nature 12 (12): 2012

9 Wu WH, "Sesame ingestion affects sex hormones, antioxidant status, and blood lipids in postmenopausal women" 136 (136): 1270-1275, 2006

10 Song I, "Regulatory mechanism of NFATc1 in RANKL-induced osteoclast activation" 583 (583): 2435-2440, 2009

11 Roodman GD, "Regulation of osteoclast differentiation" 1068 : 100-109, 2006

12 Zawawi M, "Regulation of ITAM adaptor molecules and their receptors by inhibition of calcineurin-NFAT signalling during late stage osteoclast differentiation" 427 (427): 404-409, 2012

13 Boulbaroud S, "Preventive effects of flaxseed and sesame oil on bone loss in ovariectomized rats" 11 (11): 1696-1701, 2008

14 Touaitahuata H, "Podosomes are dispensable for osteoclast differentiation and migration" 92 (92): 139-149, 2013

15 El Wakf AM, "Osteoprotective effect of soybean and sesame oils in ovariectomized rats via estrogen-like mechanism" 66 (66): 335-343, 2014

16 Matsuzaki K, "Osteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cell cultures" 246 (246): 199-204, 1998

17 Boyle WJ, "Osteoclast differentiation and activation" 423 (423): 337-342, 2003

18 Nakashima T, "New insights into osteoclastogenic signaling mechanisms" 23 (23): 582-590, 2012

19 Kim K, "NFATc1 induces osteoclast fusion via up-regulation of Atp6v0d2 and the dendritic cell-specific transmembrane protein (DC-STAMP)" 22 (22): 176-185, 2008

20 Kim MS, "MCP-1 is induced by receptor activator of nuclear factor-{kappa}B ligand, promotes human osteoclast fusion, and rescues granulocyte macrophage colonystimulating factor suppression of osteoclast formation" 280 (280): 16163-16169, 2005

21 Kim MS, "Induction of chemokines and chemokine receptors CCR2b and CCR4 in authentic human osteoclasts differentiated with RANKL and osteoclast like cells differentiated by MCP-1 and RANTES" 97 (97): 512-518, 2006

22 Mira Susa, "Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay" Springer Nature 2 (2): 6-, 2004

23 Kim HJ, "Glucocorticoids suppress bone formation via the osteoclast" 116 (116): 2152-2160, 2006

24 Grey A, "Evidence for a functional association between phosphatidylinositol 3-kinase and c-src in the spreading response of osteoclasts to colonystimulating factor-1" 141 (141): 2129-2138, 2000

25 Yongzheng He, "Erk1 Positively Regulates Osteoclast Differentiation and Bone Resorptive Activity" Public Library of Science (PLoS) 6 (6): e24780-, 2011

26 Chen M, "Emerging therapeutic targets for osteoporosis treatment" 18 (18): 817-831, 2014

27 Umeda-Sawada R, "Effects of sesamin and capsaicin on the mRNA expressions of delta6 and delta5 desaturases in rat primary cultured hepatocytes" 49 (49): 442-446, 2003

28 Ishii T, "Control of osteoclast precursor migration: a novel point of control for osteoclastogenesis and bone homeostasis" 7 (7): 279-286, 2010

29 Phitak T, "Chondroprotective and anti-inflammatory effects of sesamin" 80 : 77-88, 2012

30 Gilchrist A, "Chemokines and Bone" 13 (13): 61-82, 2015

31 Wilson SR, "Cathepsin K activity-dependent regulation of osteoclast actin ring formation and bone resorption" 284 (284): 2584-2592, 2009

32 Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method" 25 (25): 402-408, 2001

33 Quinn JM, "A combination of osteoclast differentiation factor and macrophage-colony stimulating factor is sufficient for both human and mouse osteoclast formation in vitro" 139 (139): 4424-4427, 1998