Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba)

다국어 초록 (Multilingual Abstract)

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza-2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

목차 (Table of Contents)

• Materials and Methods Results Discussion

참고문헌 (Reference)

1 Alghamdi, S. S, "Varietal identification and genetic purity assessment of F1 hybrid seeds using RAPD markers in faba bean (Vicia faba L.)" 829 : 269-274, 2009

2 Rubio, J., "Two genes and linked RAPD markers involved in resistance to Fusarium oxysporum f. sp. ciceris race 0 in chickpea" 122 : 188-191, 2003

3 Nyamsuren, O., "Transcriptional profiling of Medicago truncatula roots after infection with Aphanomyces euteiches (oomycota) identifies novel genes upregulated during this pathogenic interaction" 63 : 17-26, 2003

4 Snyder, W. C, "The species concept in Fusarium" 27 : 64-67, 1940

5 Gerlach, W, "The genus Fusarium: a pictorial atlas" Bundesanst. Land-Forstwirtsch 1-406, 1982

6 Booth, C, "The genus Fusarium" Commonwealth Agricultural Bureaux (for the) Commonwealth Mycological Institute 237-, 1971

7 Mantel, N, "The detection of disease clustering and a generalized regression approach" 27 : 209-220, 1967

8 Abdel Rehim, M. A, "Studies on the organisms causing root rot and wilt of horse beans (Vicia faba var. equina)" Alexandria University 1962

9 Hao, Q., "Studies on Paeonia cultivars and hybrids identification based on SRAP analysis" 145 : 38-47, 2008

10 Kumari, Y. S. M. A. I, "Stimulation of defense enzymes in tomato (Solanum lycopersicum L.) and chilli (Capsicum annuum L.) in response to exogenous application of different chemical elicitors" 5 : 10-15, 2017

11 Gomez, K. A, "Statistical procedures for agricultural research" John Wiley and Sons 704-, 1984

12 Rohlf, F. J, "Statistical power comparisons among alternative morphometric methods" 111 : 463-478, 2000

13 Li, G, "Sequence-related amplified polymorphism (SRAP), a new marker system based on a sample PCR reaction: its application to mapping and gene tagging in Brassica" 103 : 455-461, 2001

14 Elwakil, M. A., "Seed-borne pathogens of faba bean in Egypt: detection and pathogenicity" 8 : 90-97, 2009

15 Bebeli, P, "Seed science and technology" ICARDA 161-172, 1993

16 Yüzbaşioğlu, E., "Seed protein diversity among lentil cultivars" 52 : 126-128, 2008

17 Mace, E. S., "SSR analysis of cultivated groundnut(Arachis hypogaea L. )germplasm resistant to rust and late leaf spot diseases" 152 : 317-330, 2006

18 Amer F. Mahmoud, "Resistance Potential of Bread Wheat Genotypes Against Yellow Rust Disease Under Egyptian Climate" 한국식물병리학회 31 (31): 402-413, 2015

19 Murray, M. G, "Rapid isolation of high molecular weight plant DNA" 8 : 4321-4325, 1980

20 Tanksley, S. D., "RFLP mapping in plant breeding: new tools for old science" 7 : 257-264, 1989

21 Colditz, F., "Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches" 55 : 109-120, 2004

22 Shen, S. H., "Primary sequence of the glucanase gene from Oerskovia xanthineolytica. Expression and purification of the enzyme from Escherichia coli" 266 : 1058-1063, 1991

23 Powell, W., "Polymorphism revealed by simple sequence repeats" 1 : 215-222, 1996

24 Mofunanya, A., "Physiological and biochemical changes in Cucurbita moschata Duch. Ex. Poir inoculated with a Nigerian strain of moroccan watermelon mosaic virus (MWMV): Lagenaria breviflora isolate" 6 : 36-47, 2015

25 Neog, B., "Peroxidase, polyphenol oxidase and acid phosphatase activities in the stigma-style tissue of Camellia sinensis (L) O. Kuntze following compatible and incompatible pollination" 84 : 47-52, 2004

26 Abdul Wahid, O. A., "Occurrence of soil suppressiveness to Fusarium wilt disease of broad bean in Ismailia Governorate" 146 : 431-435, 1998

27 Balkaya, A, "Morphological properties of cultivar nominate selected Black Sea Region bean populations and identification by protein markers" 9 : 182-188, 2002

28 Khalaf, A. E. A., "Molecular markers for resistance of chocolate spot disease in faba bean (Vicia faba L.) using ISSR-PCR" 23 : 558-567, 2015

29 Alghamdi, S. S., "Molecular diversity assessment using sequence related amplified polymorphism (SRAP) markers in Vicia faba L" 13 : 16457-16471, 2012

30 Mondal, S., "Molecular diversity and association of SSR markers to rust and late leaf spot resistance in cultivated groundnut (Arachis hypogaea L.)" 129 : 68-71, 2010

31 Budak, H., "Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers" 108 : 328-334, 2004

32 Tekalign, A., "Molecular analysis for genetic diversity and population structure of Ethiopian faba bean (Vicia faba L.) accessions" 1 : 1010-, 2019

33 Parmar, P. P., "Microsatellite marker associated with fusarium wilt resistance in tomato" 5 : 389-393, 2009

34 Dice, L. R, "Measures of the amount of ecologic association between species" 26 : 297-302, 1945

35 Ghislain, M. D., "Marker-assisted sampling of the cultivated Andean potato Solanum phureja collection using RAPD markers" 46 : 547-555, 1999

36 Abou-Zeid, N. M., "Major organisms causing root-rot, wilt and their relative importance on faba bean, lentil and chickpea" 75 : 529-542, 1997

37 Prasad, P., "Isozymes variability among Fusarium udum resistant cultivars of pigeonpea (Cajanus cajan (L.)(Millsp)" 25 : 221-228, 2003

38 Alghamdi, S. S., "Inter-simple sequence repeat (ISSR)-based diversity assessment among faba bean genotypes" 62 : 755-760, 2011

39 Reddy, M. P., "Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding" 128 : 9-17, 2002

40 Kamboj, R. K., "Inheritance of resistance to Fusarium wilt in Indian lentil germplasm (Lens culinaris Medik.)" 50 : 113-117, 1990

41 El-Khallal, S. M, "Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (Jasmonic acid & Salicylic acid): 2-changes in the antioxidant enzymes, phenolic compounds and pathogen related- proteins" 1 : 717-732, 2007

42 Rabey, J. M., "Improvement of parkinsonian features correlate with high plasma levodopa values after broad bean (Vicia faba)consumption" 55 : 725-727, 1992

43 Mahmoud, A. F., "Identification of charcoal rot resistance QTLs in sorghum using association and in silico analyses" 59 : 243-251, 2018

44 Souframanien, J, "ISSR and SCAR markers linked to the mungbean yellow mosaic virus (MYMV) resistance gene in blackgram (Vigna mungo (L.)Hepper)" 125 : 619-622, 2006

45 Yang, T., "High-throughput novel microsatellite marker of faba bean via next generation sequencing" 13 : 602-, 2012

46 Zietkiewicz, E., "Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification" 20 : 176-183, 1994

47 Okiror, M. A, "Genetics of resistance to Fusarium udum in pigeonpea (Cajanus cajan (L.) Millsp.)" 62 : 218-220, 2002

48 Ghafoor, A., "Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDSPAGE" 123 : 367-378, 2002

49 El-Sayed, Z. G, "Genetic markers for some important traits in faba bean (Vicia faba L.)" Ain Shams University 2006

50 Nagaraja, N. R., "Genetic diversity studies and screening for Fusarium wilt (Fusarium udum Butler) resistance in wild pigeonpea accessions, Cajanus scarabaeoides (L.) Thouars" 29 : 121-129, 2016

51 Singh, A. K., "Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum)resistance in cultivated pigeonpea (Cajanus cajan)" 92 : 273-280, 2013

52 Lioi, L., "Genetic diversity of some surviving on-farm Italian common bean (Phaseolus vulgaris L.) landraces" 124 : 576-581, 2005

53 Yahia, Y., "Genetic diversity of Vicia faba L. based on random amplified polymorphic DNA and simple sequence repeat markers" 161 : 151-158, 2014

54 Tufan, H, "Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats" 31 : 29-35, 2017

55 Zeid, M., "Genetic diversity in recent elite faba bean lines using AFLP markers" 107 : 1304-1314, 2003

56 Abdel-Razzak, H. S., "Genetic diversity in faba bean (Vicia faba L.) using inter-simple sequence repeat (ISSR) markers and protein analysis" 9 : 497-503, 2012

57 Terzopoulos, P. J, "Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers" 108 : 39-44, 2008

58 Rashid, K. Y, "Genetic diversity among isolates of Rhizoctonia solani and sources of resistance in Vicia faba" 15 : 23-28, 1993

59 Amer F. Mahmoud, "Genetic Variation and Biological Control of Fusarium graminearum Isolated from Wheat in Assiut-Egypt" 한국식물병리학회 32 (32): 145-156, 2016

60 Eujayl, I., "Fusarium vascular wilt of lentil: inheritance and identification of DNA markers for resistance" 117 : 497-499, 1998

61 Duc, G, "Faba bean(Vicia faba L)" 53 : 99-109, 1997

62 Dong, Y., "Faba bean Fusarium wilt (Fusarium oxysporum) control and its mechanism in different wheat varieties and faba bean intercropping system" 25 : 1979-1987, 2014

63 Ali, M. B., "Evaluation of faba bean genotypes for yield and resistance to Fusarium root rot under greenhouse and field conditions" 14 : 374-385, 2019

64 Mahmoud, A. F, "Evaluation of certain antagonistic fungal species for biological control of faba bean wilt disease incited by Fusarium oxysporum" 3 : 1-14, 2016

65 Vandemark, G. J., "Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms" 152 : 9-16, 2006

66 Guikema, J. A, "Electrophoretic profiles of cyanobacterial membrane polypeptides showing heme-dependent peroxidase activity" 637 : 189-201, 1981

67 Jhang, T., "Efficiency of different marker systems for molecular characterization of subtropical carrot germplasm" 148 : 171-181, 2010

68 Velasco-Ramírez, A. P., "Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.)" 17 : 65-71, 2014

69 Ahmed, H. F. S., "Differential effect of jasmonic acid on the defense of faba bean against Fusarium wilt: modulation of other phytohormones and simple phenols" 4 : 447-453, 2002

70 Giovannelli, J. L., "Development of sequence characterized amplified region markers linked to downy mildew resistance in broccoli" 127 : 597-601, 2002

71 Srivastava, R. K., "Development of a coupling-phase SCAR marker linked to the powdery mildew resistance gene ‘er1’ in pea (Pisum sativum L.)" 186 : 856-866, 2012

72 Dhole, V. J, "Development of a SCAR marker linked with a MYMV resistance gene in mungbean (Vigna radiata L. Wilczek)" 132 : 127-132, 2013

73 Singh, M., "Development of RAPD markers linked to Fusarium wilt resistance gene in castor bean (Ricinus communis L.)" 28 : 1-9, 2011

74 Johnson, R., "Development in plant pathology. Vol. 1. Breeding for disease resistance" Kluwer Academic 206-, 1992

75 Saikia, R., "Detection of pathogenesis-related proteins-chitinase and β-1,3-glucanase in induced chickpea" 89 : 659-663, 2005

76 Gupta, V. K., "Current status of Fusarium wilt disease of guava (Psidium guajava L.) in India" 9 : 176-195, 2010

77 Mazen, M. M., "Cultural filtrate of Rhizobium spp. and arbuscular mycorrhiza are potential biological control agents against root rot fungal diseases of faba bean" 3 : 32-41, 2008

78 Provan J., "Copia-SSR: a simple marker technique which can be used on total genomic DNA" 42 : 363-366, 1999

79 Costa, R., "Comparison of RAPD, ISSR, and AFLP molecular markers to reveal and classify Orchard grass (Dactylis glomerata L.) germplasm variations" 11 : e0152972-, 2016

80 Amar, M. H, "Comparative genetic study among Origanum L. plants grown in Egypt" 3 : 208-222, 2013

81 Laemmli, U. K, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" 227 : 680-685, 1970

82 Almagro, L., "Class III peroxidases in plant defence reactions" 60 : 377-390, 2009

83 Alghamdi, S. S, "Chemical composition of faba bean (Vicia faba L.) genotypes under various water regimes" 8 : 477-482, 2009

84 Wang, Z., "Assessment of genetic diversity in Galega officinalis L. using ISSR and SRAP markers" 59 : 865-873, 2012

85 Ammar, M. H., "Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers" 22 : 340-350, 2015

86 Sahoo, M. R., "Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance" 163 : 241-248, 2007

87 Varshney, R. K., "Advances in cereal genomics and applications in crop breeding" 24 : 490-499, 2006

88 Prevost, A, "A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars" 98 : 107-112, 1999

89 Kleczewski, N. M, "A diagnostic guide for Fusarium wilt of watermelon" 12 : 27-, 2011