In the course of an intensive screening for inhibitors of aminopeptidase M, a peptide-like inhibitor was isolated from an actinomycete strain, which was identified as Streptomyces sp, SL387. The yield improvement and fermentation studies of this strai...
In the course of an intensive screening for inhibitors of aminopeptidase M, a peptide-like inhibitor was isolated from an actinomycete strain, which was identified as Streptomyces sp, SL387. The yield improvement and fermentation studies of this strain were also done for obtaining a sufficient amount of substances for chemical studies and for biological evaluation of inhibitor. This inhibitor was purified by use of column chromatography of Amberlite XAD-2, Dove-ex 50, DEAF-cellulose, MCI gel, Sephadex LH-20, Europrep 60-60 C_(18)(ODS) gel, and then isolated through HPLC as colorless powder. The purified inhibitor was competitive with substrate, and IC_(50) of it was 0.05 ㎍/㎖.