한국산 생약으로부터 해독물질의 개발(제3보) 카드뮴으로 처리한 3T3섬유모세포에서 소엽 메탄올 분획의 수복효과 = Development of Antitoxic Agents from Korean Medicinal Plants (Part 3) Repaired Effects of Methanol Fraction of Perilla Frutescens on 3T3 Fibroblast Treated with Cadmium

다국어 초록 (Multilingual Abstract)

This study was carried out to evaluate the cytotoxicity of cadmium on 3T3 fibroblast and to develop the antidote on 3T3 fibroblast which was injuried by $IC_{50}$ of cadmium. The groups for repaired effects were divided into 7 groups such as medium alone treated group. Cadmium treated $IC_{50}$ groups and 5 experimental groups $(IC_{50}$ cadmium plus $10^{-4}$ concentration of each methanol fraction). After incubation for 48 hrs in the same conditions, MTT (tetrazolium MTT), NR (neutral red) and SRB (sulforhodamine B protein) assay were measured. Light microscopic observations were also investigated. The ethyl acetate fraction of Perilla frutescens showed significantly repaired effect against cadmium cytotoxiclty and this fraction inhibited critical cell regeneration in light microscopy.