Presence of autocrine hepatocyte growth factor-Met signaling and its role in proliferation and migration of SNU-484 gastric cancer cell line

다국어 초록 (Multilingual Abstract)

Autocrine stimulation via coexpression of hepato-cyte growth factor (HGF) and its receptor (Met) has been reported in many human sarcomas, but few in carcinomas. In this report, we found that one gastric cancer cell line, SNU-484, among 11 gastric cell lines tested has an autocrine HGF- Met stimulation. RT-PCR, ELISA and scatering assay using MDCK cells revealed that SNU-484 cels secreted a significant amount of active HGF (about 1.250.41 ng/24 h/106tioned medium. Resultantly, Met in this cell line was constitutively phosphorylated. Neutralizing antibodies against HGF reduced the tyrosine phosphorylation of Met, resulting in the inhibition of cell proliferation and migration (P＜ 0.005). To the best of our knowledge, this is the first report on autocrine HGF-Met signaling in a gastric cancer cell line. Our observations with SNU-484 cels sugest that HGF is involved in the deve-lopment and/or progresion of some gastric car-cinoma through an autocrine mechanism.

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