纖維素 分解能을 가진 酵母에 관한 硏究 = Studies on the cellulolytic yeasts

다국어 초록 (Multilingual Abstract)

Two strains of cellulytic yeats were isolated fran water body in the midstream of the Yeoung San River and the Physiological characteristics of theirs as well as their CMCase activity was investigated. The results are as follows:
1. The optimum temperature for growth of the two isolatates was 20 ˚C and they seemed to be psychrophilic.
Furthermore, they had a strong cellobiose-utilizing ability.
In view ofmprphological features, the present isolates appeared to be more closely related to Trichosporon cutaneum, but there was a difference in the assimilation of KNO_3 and NaNO_3 between them. Since the morphological and physiological features are identical to those of Trichosporon pullulans, the present two isolates were classified as Tr. pullulans.
2. The optimum pH for CMCase activity From both Tr. pullulans 153 and 198 strains was 5.0 and their stability on pH ranged from pH 5.0 to 7.0.
3. The optimum temperature of the above two enzymes activity was 30 ˚C, and after the enzymes were heated at various temperatures for I hour, enzyme activity showed the best stability at 30 -40˚C and the rapid decrease at 60˚ and 80˚C
4. When the enzyme activities for Tr. pullulans 153 and 198 strains were estimated as total reducing sugar produced at the same time, the former showed greater CMCase acitivity than the latter.
5. The enzyme activity of above two strains was increased in proportion to the inclement in concentration of substrate.

목차 (Table of Contents)

• 목차
• ABSTRACT = 1
• 緖論 = 3
• 材料 및 方法 = 4
• 1. 分離 및 培養基保存 = 4
• 2. 使用한 纖維素 基質 = 4
• 3. 粗酵素의 調製 = 4
• 4. 粗酵素의 還元生成 = 5
• 5. 酵素活性의 條件 = 5
• 結果 및 考察 = 8
• 摘要 = 14
• REFERENCE = 16