慶北 北部地域 動物 및 사람으로부터 分離된 Staphylococcus aureus 菌의 抗菌劑 耐性과 分子生物學的 特性 = Antimicrobial Resistance and Molecular Biological Characteristics of Staphylococcus aureus Isolated from Animals and Human in a Northern Area of Gyeongbuk Province

다국어 초록 (Multilingual Abstract)

This studies was conducted to investigate antimicrobial resistance and molecular biological characteristics of Staphylococcus aureus (S. aureus) from animals and S. aureus from human in a northern areas of Gyeongbuk province.
The first study was conducted to investigate the antimicrobial resistance of S. aureus isolated from animals (bovine mastitic milk, chickens, Korean native cattle, Korean native goats, pigs, dog and mouse) in a northern areas of Gyeongbuk province. A total of 149 strains of S. aureus were isolated from bovine mastitic milk (80 strains), chickens (36 strains), Korean native cattle (17 strains), mouse (10 strains), Korean native goats (3 strians), pigs (2 strians), dog (1 strain). As a results of antimicrobial resistance test by using 14 antimicrobial agents, all isolates (17 strains) from Korean native cattle was resistant to penicillin and ampicillin, 88% to lincomycin, 76% to tetracycline. 58.8% of isolates from bovine mastitic milk were shown resistance to penicillin and ampicillin, 19% to gentamicin, 6% to tetracycline, respectively. Two methicillin resistant S. aureus (MRSA) were isolated from bovine mastitic milk and revealed the multi-drug resistance. 72% of isolates from chicken were shown resistance to tetracycline, 58% to penicillin and ampicillin, 42% to streptomycin, 31% to lincomycin, 25% to norfloxacin, 22% to gentamicin, in order. As a results of CVR test, most of S. aureus isolates (98.7%) from animals were SR types. As a results of REP-PCR, there were difference between S. aureus from animals and S. aureus from human in genetic level.
In second study, 114 strains of S. aureus isolates were isolated from human clinical specimens. Antimicrobial resistance test and mec A gene PCR showed that 61.7% of the S. aureus isolated from human were MRSA. 71.9% of isolates showed resistance to tetracycline, 53.5% to ciprofloxacin, 68.4% to gentamicin. But all isolates showed no resistance to vancomycin. All MRSA isolates had more than 5 drug resistance patterns. But 9.1%(4/44) of MSSA showed more than 5 drug resistance patterns. The results of coagulase PCR-RFLP for MRSA isolates showed 5 types. Type A and type B were the major colonal strains which comprized of 70.4%(50 strains) and 15.5%(11 strains), respectively and other types were in small numbers. As a results of PFGE analysis for 30 strains of S. aureus (27 strains of MRSA fron human, 2 strains of MSSA from human, and 1 strain of MRSA from bovine mastitis), there was no relationship between one MRSA from bovine mastitis and 27 strains of MRSA from human. As a results of CVR test, 59.6% of isolates were SR type, 32.5% WR type, and 7.9% DR type. This result showed that there were difference in CVR test between S. aureus from animals and S. aureus from human.
In third study, 40 strains of S. aureus were isolated bovine mastic milk at a dairy farm in Yeong-ju city, Gyeongbuk province. As a results of antimicrobial resistance test, the S. aureus revealed 55% of isolates were resistant to ampicillin and penicillin, 7.5% to gentamicin. But 45% of isolates were sensitive to all antimicrobial agents used. In case of enterotoxin production, 56.3% of isolates produced enterotoxin D. Two strains of enterotoxin D producers produced both enterotoxin B and D. As a results of enterotoxin multiplex PCR for 9 strains which had enterotoxin genes, the 9 strains also produced enterotoxin in vivo test using suckling mouse assay. According to isolation date, 15 representative strains were selected. As a results of PFGE analysis of the 15 representative strains, 14 strains were identical. Therefore I consider the identical strains of S. aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis by S. aureus.
As a general conclusion, difference between S. aureus from animals and S. aureus from human were as followed. In case of antimicrobial resistance, S. aureus from animals had about 60% resistance to penicillin. But S. aureus from human had 100% resistance to it. Among 114 strains of S. aureus from human, 74 strains (64.9%) showed more than 5 drug resistance. But among 149 strains of S. aureus from animals, only 5 strains (3.4%) showed more than 5 drug resistance. 61.7% of S. aureus from human were MRSA. But only 1.3% of S. aureus from animals were MRSA. As a results of PFGE analysis, There were no relationship between 27 strains of MRSA from human and 1 strain of MRSA from bovine mastitic milk. As a results of CVR test analysis, There were much difference between S. aureus from human and S. aureus from animals. As a results of REP-PCR, there were difference in genetic characteristics between S. aureus from human and S. aureus from animals.

목차 (Table of Contents)

• 목차
• Ⅰ.총괄 서론 = 1
• Ⅱ.제1장 경북 북부지역 동물에서 분리된 Staphylococcus aureus의 약제내성 조사 및 MRSA 균주의 검출
• 1.서론 = 4
• 2. 재료 및 방법 = 8
• 3. 결과 = 17
• 4. 고찰 = 34
• 5. 요약 = 42
• 제2장 경북 북부지역의 한 종합병원에서 분리된 Staphylococcus aureus의 항균제 내성과 PCR-RFLP를 이용한 MRSA의 Molecular Typing
• 1.서론 = 44
• 2.재료 및 방법 = 47
• 3.결과 = 54
• 4.고찰 = 70
• 5.요약 = 77
• 제3장 젖소 유방염으로부터 분리된 Staphylococcus aureus의 분자생물학적 Typing과 Multiplex PCR을 이용한 장독소 검출
• 1.서론 = 79
• 2.재료 및 방법 = 82
• 3.결과 = 88
• 4.고찰 = 96
• 5.요약 = 99
• Ⅲ.총괄 결론 = 100
• Ⅳ.참고문헌 = 103
• Abstract = 122