6가크롬에 노출된 랫트 뇌기저핵의 유전자 발현 분석 = Gene Expression Profile in Basal Ganglia of Chromium(VI) Exposed Rat Based on cDNA Experession Array

다국어 초록 (Multilingual Abstract)

This study aimed at investigating the gene expression profile in basal ganglia of hexa-valence chromium exposed rat based on cDNA array analysis. For cDNA array, Sprague-Dawley male rats (300 ± 25 g) were administrated with 15 mg/kg BW/day of potassium dichromate by gavage (0.3 mIl dissolved in saline for 10 days (n=5). For dose-related gene expression analysis. rats were administrated with 0, 1, 5 mg/kg BW/day of potassium dichromate for 10 days. Control rats were administrated with equal volume of saline (n=5). For cDNA array analysis, RNA samples were extracted from brain tissue and reverse-transcribed in the presence of ( α32pJ-dATP. Membrane sets of the Atlas array II and Toxicology array kits were hybridized with cDNA probe sets. RT-PCR and Northern blot hybridization methods were employed for validation and assessment of the dose-related gene expression profile, respectively. Arnong the 2352 cDNAs, 43 genes showed significant (>two-fold) changes in expression. 22 genes were up-regulated and 21 genes were down-regulated in the 15 mg/kg BW/day hexa-valence chromium treated group than control. According to the Northern blot hybridization analysis, heat shock protein 47, neurodegeneration associated protein 1 and pituitary specific growth factor 1a genes were up-regulated, but Gamma-aminobutyl-acid a1 subunit, neuroligin2, brain calcium-transporting plasma membrane type ATPase genes were down-regulated even in the low-dose of hexa-valence chromium exposed group (1 mg/kg BW/day) than control. Genes that detected in this study may be closely related to the hexa-valence chromium-induced neurotoxicity in the rat basal ganglia and addition study of these genes can give some more useful information about the neuro-toxic mechanism by hexa-valence chromium.

목차 (Table of Contents)

• 1. 서 론
• 2. 연구 재료 및 방법
• 3. 연구 결과
• 4. 고찰
• 5. 결론

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