Analysis of Photobiomodulation Effects in Cardiac Hypertrophy in Experimental Model of Myocardial Infarction

다국어 초록 (Multilingual Abstract)

The process of cardiac remodeling is characterized by changes in geometry, volume, mass and constitution of the heart in response to tissue aggression. Myocardial hypertrophy is an important component of cardiac remodeling, which allows the heart to maintain its basic functions under increasing load conditions. The renin‐angiotensin system plays an important role in the pathophysiology of myocardial infarction and cardiac hypertrophy and contributes to the progression of heart failure.
To evaluate the effect of photobiomodulation in genes participating in the cardiac hypertrophy process, by the analysis of Edn1, Ace, Agtr1a and Ace2 mRNA.
Fifteen female Wistar rats were divided into three groups: Sham (5), IM (5) and IM + Laser (5) (Ethics Committee: 0015–2012). The infarction induction was made by occlusion of the descending left coronary artery, the laser being applied for 60 seconds at the occlusion site immediately after the infarction. Left ventricle samples from the experimental animals were collected 72h after infarction. Modifications of the gene expression of the genes: Edn1, Ace, Agtr1a and Ace2 were analyzed by Real‐Time PCR.
The IM group determined a significant increase in expression of the genes: Edn1, Ace and Agtr1a when compared to the Sham group 3 days after the infarction. Laser therapy significantly reduced the expression of these genes when compared to the IM group: Edn1, Ace and Agtr1a when compared to the IM group, suggesting a reduction in hypertrophic processes after laser therapy.
Increased ACE expression in experimental models of myocardial infarction, as well as increased endothelin, are related to hypertrophic processes after myocardial infarction. Our studies suggest the action of the laser in the infarcted myocardium through the participation of the renin angiotensin system with the reduction of the expression of the genes involved in these processes.
Support or Funding Information
FAPESP, CNPq, CAPES, UNINOVE
This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.