골다공증 치료제로 이용되고 있는 hPTH는 체내의 혈중 칼슘 농도를 조절하는 인간의 부갑상선 호르몬이다. 본 연구에서는 hPTH를 효율적으로 생산하는 돼지세포를 구축하고자 다양한 retrovirus ...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A75265257
2004
Korean
KCI등재후보,01
학술저널
197-202(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
골다공증 치료제로 이용되고 있는 hPTH는 체내의 혈중 칼슘 농도를 조절하는 인간의 부갑상선 호르몬이다. 본 연구에서는 hPTH를 효율적으로 생산하는 돼지세포를 구축하고자 다양한 retrovirus ...
골다공증 치료제로 이용되고 있는 hPTH는 체내의 혈중 칼슘 농도를 조절하는 인간의 부갑상선 호르몬이다. 본 연구에서는 hPTH를 효율적으로 생산하는 돼지세포를 구축하고자 다양한 retrovirus vector를 이용하였는데 다음과 같은 결과를 관찰하였다. 1) hPTH 유전자의 전이를 확인하기 위해 RT-PCR을 수행한 결과, 형질전환된 모든 돼지세포에서 420 bp의 hPTH에 해당하는 단편을 확인할 수 있었으며, WPRE가 도입되지 않은 실험군보다 WPRE서열이 도입된 실험군에서 더 강한 밴드를 확인하였다. 2) ECLIA 측정 결과 hPTH 유전자가 도입된 모든 세포에서 hPTH가 생성되었으며, 특히 Tet-On system에서는 doxycycline을 처리한 실험군에서 hPTH의 발현이 유도되었음을 확인하였다. 또한 RT-PCR과 ECLIA을 수행하여 hPTH의 도입 여부와 단백질 생산을 비교한 결과, WPRE 서열이 hPTH 유전자의 downstream 위치에 도입된 실험군에서 가장 많은 단백질이 생산됨을 확인할 수 있었다.
다국어 초록 (Multilingual Abstract)
Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis....
Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis. By using retrovirus vectors carrying tetracycline-regulatory expression system and WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, we could control PTH expression with tetracycline and boost the promoter activity. Considering that low or constitutive expression of the transgene has been one of major problems that needs to be solved in transgenic animal studies, our results could be helpful in successful production of transgenic pigs as bioreactors.
목차 (Table of Contents)
참고문헌 (Reference)
1 "What is osteoporosis?." 79 : 133-138, 2003
2 "Transcriptional activation by tetracyclines in mammalian cells." 268 : 1766-1769, 1995
3 "Tight control of gene expression in mammalian cells by tetracycline- responsive promoters. Proc Natl Acad Sci USA 89" 5547-5551, 1992
4 "Tetracycline-controlled transcriptional regulation systems: advances and application in transgenic animal modeling. Semin Cell Dev Biol" 13 : 121-128, 2002
5 "Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum Gene Ther" 9 : 1939-1950, 1998
6 "Structure of human parathyroid hormone 1-37 in solution. J Biol Chem" 270 : 15194-15202, 1995
7 "Retrovirus vector-mediated Gene Transfer into the Fertilized Embryos of the Farm Animals. Animal Reprod." 19 : 293-305, 1995
8 "Retrovirus variation and evolution. Genome 31" 17-22, 1989
9 "Parathyroid hormone secretion in chronic human endogenous hypercortisolism. Braz J Med Biol Res" 35 : 229-236, 2002
10 "PTH restores bone mass and enhances osteoblast IGF-1 gene expression ovariectomized rats. Bone" 16 : 357-365, 1995
1 "What is osteoporosis?." 79 : 133-138, 2003
2 "Transcriptional activation by tetracyclines in mammalian cells." 268 : 1766-1769, 1995
3 "Tight control of gene expression in mammalian cells by tetracycline- responsive promoters. Proc Natl Acad Sci USA 89" 5547-5551, 1992
4 "Tetracycline-controlled transcriptional regulation systems: advances and application in transgenic animal modeling. Semin Cell Dev Biol" 13 : 121-128, 2002
5 "Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum Gene Ther" 9 : 1939-1950, 1998
6 "Structure of human parathyroid hormone 1-37 in solution. J Biol Chem" 270 : 15194-15202, 1995
7 "Retrovirus vector-mediated Gene Transfer into the Fertilized Embryos of the Farm Animals. Animal Reprod." 19 : 293-305, 1995
8 "Retrovirus variation and evolution. Genome 31" 17-22, 1989
9 "Parathyroid hormone secretion in chronic human endogenous hypercortisolism. Braz J Med Biol Res" 35 : 229-236, 2002
10 "PTH restores bone mass and enhances osteoblast IGF-1 gene expression ovariectomized rats. Bone" 16 : 357-365, 1995
11 "Nucleotide sequence of a cloned woodchuck hepatitis virus genome comparison with the hepatitis B virus sequence. J Virol 41" 51-65, 1982
12 "Nonmedical management of osteoporosis. Curr Opin Rheumatol" 14 : 441-446, 2002
13 "New anabolic theraphies in osteoporosis. Curr Opin Rheumatol" 14 : 433-440, 2002
14 "Long-term control of erythropoietin secretion by doxycycline in mice transplanted with engineered primary myoblasts." 3 : 299-305, 1997
15 "Kasakura S Clinical usefulness of serum PIVKA-II levels determined by ECLIA system as a tumor maker for hepatocellular carcinoma. Rinsho Byori" 46 : 936-941, 1998
16 "Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth. J Cell Sci 90" 295-300, 1988
17 "How to make tetracycline-regulated transgene expression go on and off. Anal Biochem" 309 : 79-84, 2002
18 "Generation of high-titer pseudotyped retroviral vectors with very broad host range. Methods Cell Biol 43" 99-112, 1994
19 "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA" 97 : 7963-7968, 2000
20 "Doxycycline- mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci USA" 93 : 10933-10938, 1996
21 "Discoveries, drugs and skeletal disorders" 1 : 784-796, 2002
22 "Controlled gene expression with a reverse tetracycline-regulated retroviral vector (RTRV) system. Biochem Biophys Res Commun" 234 : 769-773, 1997
23 "Control of memory formation through regulated expression of a CaMKII transgene. Science" 274 : 1678-1683, 1996
24 "Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90" burrasca (burrasca): 8033-8037, 1993
25 "Complete amino acid sequence of human parathyroid hormone. Biochemistry" 17 : 5723-5729, 1978
26 "Comparison of CMV, RSV, SV40 viral and Vλ1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochim Biophys" 1446 : 135-139, 1999
27 "CRM1- dependent function of a cis-acting RNA export element. Mol Cell Biol" 22 : 2057-2067, 2002
28 "Analytical and clinical evaluation of TSH and thyroid hormones by electrochemiluminescent immunoassays. Clin Biochem" 32 : 395-403, 1999
Deposition of Mucin Coat on Rabbit Embryos Cultured In Vitro Following Oviductal Transfer
Formation of Chimeric Gap Junction Channels in Mammalian Ovarian Follicle
Nitric Oxide 화합물 첨가가 소 체외수정란의 체외발육에 미치는 효과
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2019-03-14 | 학회명변경 | 한글명 : 한국수정란이식학회 -> 사단법인 한국동물생명공학회영문명 : The Korean Society Of Embryo Transfer -> The Korean Society of Animal Reproduction and Biotechnology | |
2016 | 평가예정 | 신규평가 신청대상 (신규평가) | |
2013-04-01 | 평가 | 탈락(현장점검) (기타) | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2006-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2005-03-21 | 학술지등록 | 한글명 : Reproductive & Developmental biology외국어명 : Reproductive & Developmental biology | |
2005-01-01 | 평가 | 등재후보학술지 유지 (등재후보1차) | |
2003-01-01 | 평가 | 등재후보학술지 선정 (신규평가) |