베타수용체의 자극제와 차단제가 가토 모양체상피세포의 미세구조에 미치는 영향에 관한 연구 = The Effect of Beta-adrenergic Agonist and Antagonists on the Rabbit Ciliary Epithelium

다국어 초록 (Multilingual Abstract)

A beta-blocking agent is now widely used in the treatment of glaucoma. The mechanism of its action has been reported to decrease aqueous humor production. The present study was undertaken to elucidate the ultrastructural changes of ciliary epithelium following topical application of beta-adrenergic agonist and antagonists.
Twelve albino rabbits (24 eyes) were used and divided into 4 groups: each group included 3 rabbits(6 eyes). Two beta-blockers (1% carteolol and 0.5% betaxolol) were instilled into the conjunctival sac of group 1 and group 2 respectively. Beta-agonist (1% isoproterenol) was instilled into the conjunctival sac of group 3. Normal saline was instilled into the conjunctival sac of control group.
The eyes were enucleated at 30 minutes and 1 hour after instillation of drugs respectively, and studied by electron microscopy.
The results were as follows:
1. Thirty minutes after instillation of carteolol, the mitochondrias of nonpigmented epithelial cells were increased in size and number, and intracytoplasmic vaculoes were shown. One hour after instillation, the mitochondrias were slightly swollen, and the intercellular spaces between the lateral interdigitation of nonpigmented epithelial cells were dilated.
2. Thirty minutes after instillation of betaxolol, intracytoplamic organelles were not significantly changed as compared with those after carteolol But one hour after instillation, the intercellular spaces between the lateral interdigitation of nonpigmented epithelial cells were dilated.
3. After instillation of isoproterenol, lateral intercellular spaces were slightly enlarged; however, these findings were returned to normal later. Thirty minutes and one hour after instillation, an obvious increase in the volume of smooth endoplasmic reticulum and dilatation of the cisternae of Golgi apparatus were observed.
Those results implicated that beta-blocking agents may suppress aqueous humor formation by morphological alterations of nonpigmented epithelial cell as compared with morphological changes following instillation of beta-stimulating agent.