A method combining the FTA Elute card and visual colorimetric loop‐mediated isothermal amplification (FTA‐e/LAMP) was tested to diagnose Streptococcus agalactiae infections in vitro and in vivo. FTA‐e/LAMP consists of two main steps: first, the ...
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https://www.riss.kr/link?id=O106358400
2021년
eng
0140-7775
1365-2761
SCI;SCIE;SCOPUS
학술저널
Journal of fish diseases
505-512 [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
A method combining the FTA Elute card and visual colorimetric loop‐mediated isothermal amplification (FTA‐e/LAMP) was tested to diagnose Streptococcus agalactiae infections in vitro and in vivo. FTA‐e/LAMP consists of two main steps: first, the ...
A method combining the FTA Elute card and visual colorimetric loop‐mediated isothermal amplification (FTA‐e/LAMP) was tested to diagnose Streptococcus agalactiae infections in vitro and in vivo. FTA‐e/LAMP consists of two main steps: first, the FTA card is used to extract DNA and then a colorimetric loop‐mediated isothermal amplification (LAMP) reaction is carried out on the extracted DNA. In vitro sensitivity was 1.9 x 102 CFU/mL, and regarding specificity, all nine S. agalactiae strains tested positive. All Streptococcus spp. tested negative, except for S. dysgalactiae, thereby indicating the need for another set of primers to distinguish this species from S. agalactiae. To diagnose S. agalactiae infections using FTA‐e/LAMP in vivo, two experimental trials on juvenile Oreochromis niloticus infected with bovine or piscine strains were carried out. Sensitivity in symptomatic fish was 100%, and 50.7% of fish without signs were positive. All negative control fish tested negative (n = 28). No bacteria were detected after 16 days post‐infection (dpi). Accuracy during the first week (1–7 dpi) was 89% and decreased to 44% thereafter (10–22 dpi). FTA‐e/LAMP results suggest that this method is a promising tool for early and fast diagnosis of S. agalactiae on tilapia farms.
The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida