Confocal and Electron Microscope Study of the Take-Up Process of Intraperitoneally-Administered Nanoparticles

다국어 초록 (Multilingual Abstract)

By using confocal laser scanning microscopy and transmission electron microscopy, we were
able to investigate the initial take-up and distribution of
uorescent nanoparticles injected into
the abdominal cavity of mice. We found that intraperitoneally administrated nanoparticles were
principally taken up by the omental fat band in the initial stage whereas absorption by other organs
was prevented by their covering membranes. The milky spots on the omental fat band, known as
peculiar lymphoid tissues, absorbed
uorescent nanoparticles within tens of minutes through their
loose boundaries and immune cells. Immunohistochemistry and electron microscopy revealed that
nanoparticles were engulfed by the immune cells in the milky spots and were transported mainly
through lymph vessels to other organs. This route could be an initial pathway for the journey to
reticular endothelial systems like the liver. The direct connection of the omental fat band to the
pancreas, the stomach and the spleen implies signicant medical applications. For example, toxic
or therapeutic eects of nanoparticle-laden drug delivery to these digestive organs are expected.

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