Induced apoptosis and anti-abnormal metastasis in cancer cells is a therapeutic strategy for the treatment of cancer. In this study, I investigated the regulatory mechanism of Torilis japonica 95% ethanol extract-induced apoptosis through the AMPK/p38...
Induced apoptosis and anti-abnormal metastasis in cancer cells is a therapeutic strategy for the treatment of cancer. In this study, I investigated the regulatory mechanism of Torilis japonica 95% ethanol extract-induced apoptosis through the AMPK/p38 MAPkinase signaling pathway and suppressed abnormal metastasis by epidermal-mesenchymal transition (EMT) through regulated EGFR signaling pathways.
My results showed that TJE induced apoptosis through generated intracellular Reactive Oxygen Species (ROS), and it responsible for depressed mitochondria membrane potential. And not only in vitro experiment, but TJE suppressed tumor growth and induced apoptosis in in vivo experiment.
In addition, treatment with TJE along with stimulation by EGF prevented changes in cell morphology, mobility, expression of actin polarization proteins and EMT markers. Using specific inhibitors and siEGFR, it was demonstrated that TJE suppressed EMT through EGFR inactivation and regulation of its downstream signaling pathways.
I suggest that TJE is a new potential multi-target cancer prevention reagent for induced apoptosis through generated intracellular ROS and EGFR-targeted anti-abnormal metastasis.