Anti-inflammatory Effect of Grape Seed may Involve the Induction of Heme Oxygenase-1 and Suppression of Nuclear Factor-κB Activation

다국어 초록 (Multilingual Abstract)

This study aimed to elucidate the antiinflammatory mechanism of grape-seed acetone extract (GSAE) in lipopolysaccharide (LPS)-stimulated RAW264.7macrophages. The GSAE at the highest concentration (500μg/mL) significantly inhibited nitric oxide production by approximately 78% and suppressed inducible nitric oxide synthase protein expression by approximately 83% compared to LPS-stimulated cells. The GSAE also suppressed inhibitor κBα phosphorylation and nuclear factor-κB (NF-κB; p65) transcriptional activity and translocation to the nucleus. In addition, GSAE induced the expression of heme oxygenase-1 (HO-1) in a dose-dependent manner,and that blocking HO-1 activity eliminated the inhibitory effects of the GSAE. The addition of carbon monoxide, a byproduct of heme degradation by HO-1, mimicked the inhibitory action of low concentrations of GSAE. These data suggest that GSAE carries out anti-inflammatory actions in macrophages through the induction of HO-1 and suppression of NF-κB activation.

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