Growth and Detachment of 5 Helix DNA Ribbons

다국어 초록 (Multilingual Abstract)

<P>We report on the concentration-dependent surface-assisted growth and time-temperature dependent detachment of one-dimensional 5 helix DNA ribbons (5HR) on a mica substrate. The growth coverage ratio was determined by varying the concentration of the 5HR strands in a test tube, and the detachment rate of 5HR on mica was determined by varying the incubation time at a fixed temperature on a heat block. The topological changes in the concentration-dependent attachment and the time-temperature-dependent detachment for 5HR on mica were observed via atomic force microscopy. The observations indicate that 5HR started to grow on mica at similar to 10 nM and provided full coverage at similar to 50 nM. In contrast 5HR at 65 degrees C started to detach from mica after 5 min and was completely removed after 10 min, The growth and detachment coverage show a sinusoidal variation in the growth ratio and a linear variation with a rate of detachment of 20%/min, respectively. The physical parameters that control the stability of the DNA structures on a given substrate should be studied to successfully integrate DNA structures for physical and chemical applications.</P>