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      HPLC에 의한 동물성 수산식품 중의 Vitamin D₃와 그 관련물질 정량에 관한 연구 = Quantification of Vitamin D₃ and Its Derivatives in Marine Animal Foods by HPLC

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      https://www.riss.kr/link?id=A103158350

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      다국어 초록 (Multilingual Abstract)

      Contents of vitamin D₃and 25-OH-vitaimin D₃in marine animal products(20 species) were determined by HPLC. The isomers of vitamin D, D₂and D₃, were not clearly separated on a reversed phase, μBonda Pak, with 20% methanol-acetonitrile, and on a normal phase, Zorbax SIL. With 0.4% isopropanolhexane, but 25-OH-vitamin D₂and D₃were separated on either μBonda Pak with 10% methanol-acetonitrile, or on Zorbax SIL with 2.2% isopropanol-hexane, respectively. Although levels of vitamin D₃and 25-OH-vitamin D₃ varied remarkably according to species, their average value(fish ; 1,187~36,007 I. U/sample 100g, mussel ; 58~1,706 I. U/sample 100g, pickle : 1,208~79.358 I. U/sample 100g) was greatly higher than that of meat(80~l00 I. U/sample 100g) and dairy products(400~800 I. U/sample 100g). Fatty tissues of fish and pickled fish intestines contained high level of vitamin D₃and 25-OH-vitamin D₃, while the clam and mussel known to have various kinds of sterol including Δ₇-sterol showed very low levels of vitamin D₃and its derivative.
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      Contents of vitamin D₃and 25-OH-vitaimin D₃in marine animal products(20 species) were determined by HPLC. The isomers of vitamin D, D₂and D₃, were not clearly separated on a reversed phase, μBonda Pak, with 20% methanol-acetonitrile, and on a...

      Contents of vitamin D₃and 25-OH-vitaimin D₃in marine animal products(20 species) were determined by HPLC. The isomers of vitamin D, D₂and D₃, were not clearly separated on a reversed phase, μBonda Pak, with 20% methanol-acetonitrile, and on a normal phase, Zorbax SIL. With 0.4% isopropanolhexane, but 25-OH-vitamin D₂and D₃were separated on either μBonda Pak with 10% methanol-acetonitrile, or on Zorbax SIL with 2.2% isopropanol-hexane, respectively. Although levels of vitamin D₃and 25-OH-vitamin D₃ varied remarkably according to species, their average value(fish ; 1,187~36,007 I. U/sample 100g, mussel ; 58~1,706 I. U/sample 100g, pickle : 1,208~79.358 I. U/sample 100g) was greatly higher than that of meat(80~l00 I. U/sample 100g) and dairy products(400~800 I. U/sample 100g). Fatty tissues of fish and pickled fish intestines contained high level of vitamin D₃and 25-OH-vitamin D₃, while the clam and mussel known to have various kinds of sterol including Δ₇-sterol showed very low levels of vitamin D₃and its derivative.

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      목차 (Table of Contents)

      • ABSTRACT
      • I. 서론
      • II. 시료 및 실험방법
      • III. 결과 및 고찰
      • IV. 결론
      • ABSTRACT
      • I. 서론
      • II. 시료 및 실험방법
      • III. 결과 및 고찰
      • IV. 결론
      • 문헌
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