감미단백질 모넬린 발현 딸기 형질전환 식물체 개발 = Development of transgenic strawberry plants expressing monellin, a sweet protein

다국어 초록 (Multilingual Abstract)

Leaf discs from ‘Yeobong’ and ‘Maehyang’ strawberry plants were used as explants for transformation.
The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in ‘Yeobong’ were higher than those of ‘Maehyang’. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants.
Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

참고문헌 (Reference)

1 조미애, "국내 딸기 재배품종의 잎절편 배양으로부터 고빈도 식물체 재생" 한국식물생명공학회 32 (32): 175-179, 2005

2 Sugaya T, "Transgenic strawberry expressing the taste-modifying protein miraculin" 25 : 329-333, 2008

3 Wang JL, "Transformation of strawberry (Fragaria ananassa Duch.)with late embryogenesis abundant protein gene" 79 : 735-738, 2004

4 Graham J, "Towards genetic based insect resistance in strawberry using the cowpea trypsin inhibitor gene" 127 : 163-173, 1995

5 Faedi W, "Strawberry breeding and varieties: situation and perspectives" 567 : 51-59, 2002

6 El Mansouri I, "Shoot regeneration and Agrobacterium-mediated transformation of Fragaria vesca L" 15 : 642-646, 1996

7 Barcelo M, "Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler" 54 : 29-36, 1998

8 Kim SH, "Redesigning a sweet protein: Increased stability and renaturability" 2 : 571-575, 1989

9 Morris JA, "Purification of monellin, the sweet principal of Dioscoreophyllum cumminsii" 261 : 114-122, 1972

10 Penarrubia L, "Production of the sweet protein monellin in transgenic plants" 10 : 561-564, 1992

11 Morgan A, "Production of herbicide tolerant strawberry through genetic engineering" 567 : 113-115, 2002

12 Houde M, "Overexpression of the acidic dehydrin WCOR410 improves freezing tolerance in transgenic strawberry leaves" 2 : 381-387, 2004

13 Gamborg OL, "Nutrient requirements of suspension culture of soybean root cells" 50 : 15-158, 1968

14 Park J-I, "Modification of sugar composition in strawberry fruit by antisense suppression of an ADPglucose pyrophosphorylase" 17 : 269-279, 2006

15 Edens L, "Microbial synthesis of the sweet tasting plant protein thaumatin" 3 : 61-64, 1985

16 Jimenez-Bermudez S, "Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene" 128 : 751-759, 2002

17 Nehra NS, "Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system" 9 : 293-298, 1990

18 Agricultural Outlook Center, "Fruit Vegetables – Strawberry" Korea Rural Economic Institute 2013

19 Qin YH, "Factors influencing the efficiency of Agrobacterium-mediated transformation in strawberry cultivar Toyonoka" 21 : 461-465, 2007

20 Ota M, "Enzymatic ligation for synthesis of single chain analogue of monellin by transglutaminase" 50 : 193-200, 1999

21 Vellicce GR, "Enhanced resistance to Botrytis cinerea mediated by the transgenic expression of the chitinase gene ch5B in strawberry" 15 : 57-68, 2006

22 Du Plessis HJ, "Efficient genetic transformation of strawberry (Fragaria ananassa Duch.) cultivar Selekta" 447 : 289-294, 1997

23 Nehra NS, "Direct shoot regeneration from strawberry leaf disks" 114 : 1014-1018, 1989

24 An G, "Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells" 81 : 86-91, 1986

25 Southern EM, "Detection of specific sequences among DNA fragments separated by gel electrophoresis" 98 : 503-517, 1975

26 Marta AE, "Breeding barriers between the cultivated strawberry, Fragaria × ananassa, and related wild germplasm" 136 : 139-150, 2004

27 Nehra NS, "Agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants" 9 : 10-13, 1990

28 Khammuang S, "Agrobacterium-mediated transformation of modified antifreeze protein gene in strawberry" 27 : 693-703, 2005

29 James DJ, "Agrobacterium mediated transformation of the cultivated strawberry (Fragaria ×ananassa Duch) using disarmed binary vectors" 69 : 79-94, 1990

30 Kyung Hee Roh, "Accumulation of Sweet Protein monellin is Regulated by the psbA 5’UTR in Tobacco Chloroplasts" 한국식물학회 49 (49): 34-43, 2006

31 Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures" 15 : 473-497, 1962