Construction of Antigen 43 System for Escherichia coli-derived Outer Membrane Particles Displaying Recombinant Antigen

다국어 초록 (Multilingual Abstract)

Antigen 43(Ag43), an autotransporter protein, consists of the N-terminal signal peptide sequence to move into inner membrane, the C-terminal β-barrel domain forming a pore in the outer membrane and the passenger domain exposed to bacterial surface. By replacing the passenger domain sequence with desired recombinant antigen, the Ag 43 system can be used to the bacterial surface display. We confirmed constructs of the β-barrel domain by deletion of the N-terminal sequence and evaluated to expose protein to the surface. It was performed by exposing eGFP to the surface, and the Ag43_700 was the most efficient construct through the flow cytometry analysis and extraction of outer membrane. This exposed platform enables the application of antigen display on outer membrane particles.