Photo-protective and Anti-melanogenic Effect from Phenolic Compound of Olive Leaf (Olea europaea L. var. Kalamata) Extracts on the Immortalized Human Keratinocytes and B16F1 Melanoma Cells

다국어 초록 (Multilingual Abstract)

Ethylacetate and butanol fractions of leaf extracts (OLE) showed the higher contents of total phenolic compounds than hexane and water fractions. Oleuropein contents were 4.21±0.57, 3.92±0.43, 0.32±0.03, 5.76±0.32, and 32.47±0.25 ㎎/100 g for ethanol extract, and hexane, chloroform, ethyl acetate, and butanol fraction, respectively. Treatment of ultraviolet-B (UVB) irradiated cells with 3 OLEs prepared by using ethylacetate and butanol at concentrations 0.001, 0.005, and 0.01% respectively showed significant recovery of cell viabilities. Treatment of dexametason 1 mM reduced tumor necrotic factor (TNF)-α secretion by about 40%. UVB irradiated immortalized human keratinocytes (HaCaT) cells were treated with 3 different OLEs at the same concentrations. Ethylacetate fraction showed the strongest inhibition activity with respect of reduction of the elevated TNF-α. Cytotoxicity of OLEs on the B16-F1 cells was evaluated through thiazolyl blue tetrazolium bromide (MTT) assay. Ethylacetate fraction has no cytotoxicity in the range of 0.005-0.01%. A slight cytotoxicity was observed at the concentration of 0.1% butanol fraction of OLE that caused 10% decrease in cell viability.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and Methods
• Results and Discussion
• References

참고문헌 (Reference)

1 최남영, "올리브 잎 분획물의 항산화기능과 아질산염 소거능력 평가" 한국식품과학회 40 (40): 257-264, 2008

2 이옥환, "올리브 잎 분획물의 항균활성 및 아질산염 소거능" 한국식품조리과학회 20 (20): 204-210, 2004

3 Pillai S, "Ultraviolet radiation and skin aging: Roles of reactive oxygen species, inflammation, and protease activation, and strategies for prevention of inflammation-induced matrix degradation-a review" 27 : 17-34, 2005

4 Rittie L, "UV-light-induced signal cascades and skin aging" 1 : 705-720, 2002

5 Kim YJ, "The anti-melanogenic effect of pycnogenol by its anti-oxidative actions" 46 : 2466-2471, 2008

6 Bashir MM, "TNF-α production in the skin" 301 : 87-91, 2008

7 Luque de Castro MD, "State-of-the-art and trends in the analysis of oleuropein and derivatives" 25 : 501-510, 2006

8 Mary FB, "Skin immune systems and inflammation: Protector of the skin or promoter of aging?" 13 : 15-19, 2008

9 Soler-Rivas C, "Review oleuropein and related compounds" 80 : 1013-1023, 2000

10 Takahiro I, "Protection of human keratinocytes from UVB-induced inflammation using root extract of lithospermum erythrorhizon" 30 : 928-934, 2007

11 Bouaziz M, "Production of antioxidants from olive processing by-products" 7 : 3231-3236, 2008

12 Skerget M, "Phenols, proanthocyanidins, flavones, and flanonols in some plant materials and their antioxidant activities" 89 : 191-198, 2005

13 Ryan D, "Phenolic compounds in olives" 123 : 31-44, 1998

14 Silva S, "Phenolic compounds and antioxidant activity of Olea europaea L. Fruits and leaves" 12 : 385-396, 2006

15 Pereira AP, "Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves" 12 : 1153-1162, 2007

16 Fisher GJ, "Pathophysiology of premature skin aging induced by ultraviolet light" 337 : 1419-1428, 1997

17 Aoki Y, "Melanogenesis inhibition by an oolong tea extract in b16 mouse melanoma cells and UV-Induced skin pigmentation in brownish guinea pigs" 71 : 1879-1885, 2007

18 Fisher GJ, "Mechanisms of photoaging and chronological skin aging" 138 : 1462-1470, 2002

19 Lam Do Phuong Uyen, "Mechanism of Skin Pigmentation" 한국생물공학회 13 (13): 383-395, 2008

20 Yoshimura M, "Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity and ultraviolet-induced pigmentation" 69 : 2368-2373, 2005

21 Seiberg M, "Inhibition of melanosome transfer results in skin lightening" 115 : 162-167, 2000

22 Shimogaki H, "In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition" 22 : 291-303, 2000

23 Annemie P, "Eicosapentaenoic acid, a n-3 polyunsaturated fatty acid differentially modulates TNF-α, IL-1α, IL-6, and PGE2 expression in UVB-irradiated human keratinocytes" 118 : 692-698, 2002

24 Teresa-Sartue M, "Effect of natural antioxidants in virgin olive oil on oxidative stability of refined, bleached, and deodorizied olive oil" 72 : 1131-1137, 1995

25 Poquet L, "Effect of dihydrocaffeic acid on UV irradiation of human keratinocyte HaCaT cells" 476 : 196-204, 2008

26 Eun-Ki Kim, "Depigmenting effect of Cinnamomum cassia Presl in B16F10 melanoma cells" 한국화학공학회 24 (24): 827-830, 2007

27 Rice-Evans CA, "Antioxidant properties of phenolic compounds" 2 : 152-159, 1997

28 Benavente-Garcia O, "Antioxidant activity of phenolics extracted from Olea europaea L" 68 : 457-462, 2000