Transformation of Mature Osteoblasts into Bone Lining Cells and RNA Sequencing-Based Transcriptome Profiling of Mouse Bone during Mechanical Unloading

다국어 초록 (Multilingual Abstract)

Background: We investigated RNA sequencing-based transcriptome profiling and the transformation of mature osteoblasts intobone lining cells (BLCs) through a lineage tracing study to better understand the effect of mechanical unloading on bone loss.
Methods: Dmp1-CreERt2(+):Rosa26R mice were injected with 1 mg of 4-hydroxy-tamoxifen three times a week starting at postnatal week 7, and subjected to a combination of botulinum toxin injection with left hindlimb tenotomy starting at postnatal week 8 to10. The animals were euthanized at postnatal weeks 8, 9, 10, and 12. We quantified the number and thickness of X-gal(+) cells onthe periosteum of the right and left femoral bones at each time point.
Results: Two weeks after unloading, a significant decrease in the number and a subtle change in the thickness of X-gal(+) cells wereobserved in the left hindlimbs compared with the right hindlimbs. At 4 weeks after unloading, the decrease in the thickness was accelerated in the left hindlimbs, although the number of labeled cells was comparable. RNA sequencing analysis showed downregulation of 315 genes in the left hindlimbs at 2 and 4 weeks after unloading. Of these, Xirp2, AMPD1, Mettl11b, NEXN, CYP2E1, Bche,Ppp1r3c, Tceal7, and Gadl1 were upregulated during osteoblastogenic/osteocytic and myogenic differentiation in vitro.
Conclusion: These findings demonstrate that mechanical unloading can accelerate the transformation of mature osteoblasts intoBLCs in the early stages of bone loss in vivo. Furthermore, some of the genes involved in this process may have a pleiotropic effecton both bone and muscle.

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