Biological Activity of Human Dimeric Hyperglycosylated Erythropoietin (dHGEPO) Fusion Proteins

목차 (Table of Contents)

• ABSTRACT
• INTRODUCTION
• MATERIALS AND METHODS
• Materials
• Construction of EPO-EPO Fusion Protein cDNA andSite-directed Mutagenesis
• Expression Vector Construction
• Cell Culture and Functional Expression
• Northern Blot Analysis
• Cell Proliferation Assay
• SDS-PAGE and Western Blot Analysis
• Enzymatic Release of N-Linked Oligosaccharides
• In Vivo Biological Activity and Pharmacokinetics Analysis
• RESULTS
• rec-hEPO Production
• Northern Blot Analysis
• Cell Proliferation Assay
• Western Blot and Treatment of N-linked Oligosaccharide
• In Vivo Biological Activity
• Pharmacokinetic Analysis
• DISCUSSION
• REFERENCES

참고문헌 (Reference)

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