Pseudomonas aeruginosa EL-KM 에 의한 환경친화적 항균물질의 생산과 특성 = Production and Characteristics of Environment-Friendly Antimicrobial Substance by Pseudomonas aeruginosa EL-KM

다국어 초록 (Multilingual Abstract)

An antimicrbial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrbial substance was 1% mannitol, 0.4% yeast extract, 0.5% NaCl, 0.2% K_2SO_4, 100μM MgSO_4·7H_2O, 10μM CaCl_2·2H_2O, 1μM FeSO_4·7H_2O, 1μM MnSO_4·4-5H_2O, initial pH 7 and 200 rpm at 30℃. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC R_f 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4℃-121℃ autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-O1 ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.