실험동물을 이용한 화분 섭취의 면역안전성과 화분을 이용한 백신의 면역원성 평가에 관한 연구 = Evaluation of immunogenicity and immunotoxicity of Lily pollen as an antigen carrier

다국어 초록 (Multilingual Abstract)

Pollen has been used for prevention or treatment of certain diseases such as diabetes, arthritis, or cancer in traditional medicine. In addition, pollen is under investigation as a host cell for a gene expression. Immunotoxicity safety of pollen intake was investigated using mice. BALB/c mice were administered with 500, 50, 5, or 0.5 mg/kg bw of lily pollen for five times a week for four weeks through gastric intubation. Comparing the control mice administered with distilled water, no significant changes were observed in body weight gain, weight of liver, spleen, and lung, and histopathological findings of liver and kidney of the mice groups administered with the pollen. Plasma level of IgG1, IgG2a, and IgE was not different among the groups. When splenic B lymphocytes were stimulated in vitro with lipopolysaccharides for 7 days, level of IgG1 and IgG2a produced in the culture supernatants was not significantly different among the groups. Furthermore, no significant alteration was observed in IL-4 and IFNγ producing ability with splenic T lymphocytes stimulated in vitro with phytohemagglutinins for 48 hours between the pollen-administered and the control mice. Immunogenicity of the pollen administered through gastric intubation was also investigated. BALB/c mice were administered with 50mg/kg bw of lily pollen expressed with Hepatitis B virus surface antigen(HBsAg, 23.1ng/g pollen) for two times a week for eight weeks through gastric intubation. The mice, which were intra-muscularly injected with sub-immunogenic dose of just prior to first pollen administration and one month after, demonstrated approximately six-fold higher HBs-antibody titer compared with that of the mice injected with the commercial vaccine alone. Overall, this study suggests that the lily pollen intake is inducing no significant modulation of humoral and cell-mediated immunity in mice and the transgenic pollen intake induce a signific elevation of HBs-antiody titer.

목차 (Table of Contents)

• I. 서 론 = 1
• II. 재료 및 방법 = 3
• 1. 화분 섭취의 면역안전성 = 3
• 1) 실험재료 = 3
• 2) 실험동물 및 화분투여 = 3
• 3) 면역안전성 평가를 위한 실험방법 = 4
• 2. 화분을 이용한 백신의 면역원성 = 6
• 1) 실험재료 = 6
• 2) 실험동물 및 화분 투여 = 6
• 3) 혈장 내 존재하는 간염 바이러스 항체의 수준을 측정 = 11
• 3. 통계처리 = 11
• III. 결과 및 고찰 = 12
• 1. 백합화분섭취의 면역안전성 측면 = 12
• 가. 백합화분 투여군과 대조군간 일반 면역병리 비교 = 12
• 나. 백합화분 투여의 체액면역에 미치는 영향 = 15
• 다. 백합화분 투여의 세포면역에 미치는 영향 = 18
• 2. 백합화분을 이용한 백신의 면역원성 = 20
• IV. 결론 = 26
• 참고문헌 = 28