Studies on the Anti-aging Action of Korean Ginseng : (Ⅰ) Comparative Study on Red and White Ginsengs on Anti-aging Action (第1報) 紅蔘과 白蔘의 老化抑制作用 比較硏究 = 高麗人蔘의 老化抑制作用에 관한 硏究

국문 초록 (Abstract)

紅蔘과 白蔘을 抗酸化作用에 의한 老化抑制作用을 究明하기 위하여 80% ethanol extract 및 水飽和부탄올로 移行한 saponin을 試料로 하여 in vitro 및 in vivo 實驗을 통하여 紅蔘과 白蔘의 老化抑制作用을 比較하였다.
HPPH에 대한 電子供與能(EDA), TBA value 및 peroxide value에 의한 過酸化脂質生成抑制作用, superoxide dismutase 및 peroxidase의 活性測定등으로 紅蔘과 白蔘의 老化抑制作用을 比較한 結果, 紅蔘은 長期間에 걸친 連續投與에서 白蔘보다 過酸化脂質生成抑制作用, superoxide dismutase 및 peroxidase의 活性을 有意性있게 增加하였다. 生體內 實驗에서 紅蔘이 白蔘보다 老化抑制作用이 현저한 것은 蒸蔘 및 加工處理中 saponin pattern의 變化에 起因하며 白蔘의 PT/PD ratio가 0.401인데 反해 紅蔘의 PT/PD ratio가 0.561로서 triol saponin이 增加되었기 때문이다.

다국어 초록 (Multilingual Abstract)

The inhibitory effects of the extract and crude saponin of red and white ginsengs on lipoperoxide formation in vitro and in vivo were studied and correlated with anti-aging. To this end, antioxidant activity, induction period and lipoperoxide were measured by the methods of EDA, POV and TBA value. And also superoxide dismutase and peroxidase activity were measured by pyrogallol autoxidation method (ΔA 420/min) and initial velocity(ΔA 436/min), respectively.
From HPLC analysis, the PT/PD ratio of red and white ginsengs was found to be 0.561% and 0.401%, respectively, and red ginseng increased the PT/PD ratio in comparison with white ginseng. The EDA activity of red ginseng was higher than that of white ginseng; red ginseng showed stronger antioxidative effect than white ginseng.
The inhibitory effect of red ginseng was lower than that of white ginseng during the induction period. It was proved that high molecular coloring substance was deeply related to the initial stage of lipoperoxidation.
There was no significant difference between red and white ginsengs in both in vitro and intraperitoneal administration experiments, and red ginseng was more effective than white ginseng in long-term administration. And also inhibitory effect on lipoperoxide formation was mainly occurred in liver, suggesting that the function of liver played an important role in anti-aging actions.
From the measurement of superoxide dismutase(SOD) activity for both ginseng groups intraperitoneally and orally administered, it was found that red ginseng group administered extract and crude saponin showed remarkable inhibitory effects in comparison with white ginseng. In particular, orally administered group showed more stronger inhibitory effect on lipid peroxidation in comparison with intraperitoneally administered group. It was also found that the continuous oral administration was more effective than temporary administration.
Red ginseng was more notable anti-aging effect in comparison with white ginseng in vivo, and this may be due to the increase of SOD activity in rat-liver. Peroxidase activity also showed similar trend to SOD activity in vitro and in vivo experiments.
Red ginseng was not only superior to white ginseng for preservation but also for biochemical and pharmaceutical efficacy.