Effects of (-)-Epigallocatechin Gallate on HMGB1 and Interleukin-1β-Mediated Barrier Disruption in Human Endothelial Cells

다국어 초록 (Multilingual Abstract)

High plasma levels of interleukin (IL)-1β and high mobility group box protein 1 (HMGB1) correlate with poor prognosis and increased mortality in patients with severe inflammation. (-)-Epigallocatechin gallate (EGCG), the main catechin in green tea,has multiple beneficial effects on reduction of inflammatory diseases; however, effects of EGCG on both IL-1β- and HMGB1-mediated barrier disruptive responses of human umbilical vein endothelial cells (HUVECs) have not been examined. Thus, effects of EGCG on IL-1β- and HMGB1-mediated barrier disruption in HUVECs were investigated. EGCG potently inhibited IL-1β or HMGB1-mediated permeability and do wn-regulated IL-1β and HMGB1-dependent adhesion and migration of THP-1 to activated HUVECs by inhibiting the activation of nuclear factor (NF)-κB and expression of tumor necrosis factor (TNF)-α. EGCG inhibited IL-1β- and HMGB1-mediated expression of cell adhesion molecules. Results showed EGCG has barrier protective activities and may represent a strategy to treat various inflammatory conditions mediated through the inhibition of IL-1β and HMGB1signaling pathways.

다국어 초록 (Multilingual Abstract)

High plasma levels of interleukin (IL)-1β and high mobility group box protein 1 (HMGB1) correlate with poor prognosis and increased mortality in patients with severe inflammation. (-)-Epigallocatechin gallate (EGCG), the main catechin in green tea,has multiple beneficial effects on reduction of inflammatory diseases; however, effects of EGCG on both IL-1β- and HMGB1-mediated barrier disruptive responses of human umbilical vein endothelial cells (HUVECs) have not been examined. Thus, effects of EGCG on IL-1β- and HMGB1-mediated barrier disruption in HUVECs were investigated. EGCG potently inhibited IL-1β or HMGB1-mediated permeability and down-regulated IL-1β and HMGB1-dependent adhesion and migration of THP-1 to activated HUVECs by inhibiting the activation of nuclear factor (NF)-κB and expression of tumor necrosis factor (TNF)-α. EGCG inhibited IL-1β- and HMGB1-mediated expression of cell adhesion molecules. Results showed EGCG has barrier protective activities and may represent a strategy to treat various inflammatory conditions mediated through the inhibition of IL-1β and HMGB1signaling pathways.

참고문헌 (Reference)

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