<P>A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DN...
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https://www.riss.kr/link?id=A107629194
2011
-
SCOPUS,SCIE
학술저널
8906-8912(7쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DN...
<P>A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.</P><P><B>Graphic Abstract</B>
<IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2011/ancham.2011.83.issue-23/ac201298r/production/images/medium/ac-2011-01298r_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac201298r'>ACS Electronic Supporting Info</A></P>
High-Fidelity Optofluidic On-Chip Sensors Using Well-Defined Gold Nanowell Crystals